ISSN:
1573-5001
Keywords:
Stereospecific assignments
;
Isotope labeling
;
Glycine
;
Proteins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A method is described for stereospecifically assigning the α-protons of glycine residues in proteins. The approach involves the stereospecific deuteration and 15N labeling of glycine and subsequent selective incorporation of this residue into the protein. The stereospecific assignments of the glycine α-protons are obtained from a comparison of a 3D 15N-resolved TOCSY spectrum of the uniformly 15N-labeled protein with a 2D/3D 15N-edited TOCSY spectrum of the protein, containing the stereospecifically deuterated and 15N-labeled glycine. The approach is demonstrated by stereospecifically assigning the glycine α-protons of the FK506 binding protein when bound to the immunosuppressant ascomycin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00179344
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