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  • Glyceraldehyde-3-phosphate dehydrogenase  (1)
  • Mutant (Nicotiana)  (1)
  • Nicotiana (nitrate reductase)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Repair in vitro of nitrate reductase-deficient tobacco mutants (cnxA) by molybdate and by molybdenum cofactor (1985)
    Springer
    Planta 163 (1985), S. 370-375 
    Details
    ISSN: 1432-2048
    Keywords: Molybdenum ; Mutant (Nicotiana) ; Nicotiana (nitrate reductase) ; Nitrate reductase mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two nitrate reductase-deficient mutant cell lines (CnxA68/2, CnxA101) of Nicotiana tabacum are shown to be repairable under in-vitro conditions by (i) molybdate or (ii) by preparations of active molybdenum cofactor of homologous or heterologous origin, thereby yielding about 20% and 80%, respectively, of the corresponding wild-type NADH-nitrate reductase (EC 1.6.6.1) activity. In-vitro repair of nitrate reductase activity is dependent on sulphydryl-group protecting reagents and ethylenediaminetetraacetic acid (EDTA) in the extraction medium, the nitrogen source in the growth medium and the age of the cells. The results support the conclusion that the cnxA gene controls the insertion of molybdenum into the molybdenum cofactor. They are consistent with the idea of two interlinked pathways for the metabolic processing of molybdenum acquisition, one involving the synthesis of the structural moiety of the molybdenum cofactor and the other involving processing of the molybdate anion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395145
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  • 2
    Electronic Resource
    Electronic Resource
    Intron-specific stimulation of anaerobic gene expression and splicing efficiency in maize cells (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 252-258 
    Details
    ISSN: 1617-4623
    Keywords: Key words Anaerobiosis ; Glyceraldehyde-3-phosphate dehydrogenase ; Introns ; Transientgene expression ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Most of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes characterized in plants and algae to date have one intron very close to the 5′ end of the gene. To study the functional relevance of some of these introns for gene expression we have analysed the influence of three 5′ introns on transient gene expression of the anaerobically inducible maize GapC4 promoter in maize cells. Under aerobic conditions, reporter gene expression is increased in the presence of the first introns of the GapC4 and GapC1 genes, and the first intron of the nuclear encoded chloroplast-specific GapA1 gene. In contrast, the GapC4 intron increases anaerobic gene expression above the level obtained for the intronless construct, while anaerobic expression of constructs harboring the GapA1 and GapC1 introns was similar to the anaerobic expression level of the intronless construct. Splicing analysis revealed that the GapC4 intron is processed more efficiently under anaerobic conditions, while no change in splicing efficiency is observed for the GapC1 and the GapA1 introns when subjected to anaerobic conditions. These results suggest that an increase in splicing efficiency contributes to the anaerobic induction of the maize GapC4 gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172925
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