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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 99 (1999), S. 305-313 
    ISSN: 1432-2242
    Keywords: Key words Binary cosmid ; BAC ; Wheat D genome ; Glutenin ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A large DNA fragment library consisting of 144 000 clones with an average insert size of 119 kb was constructed from nuclear DNA isolated from root and leaf tissue from Triticum tauschii (syn. Aegilops tauschii), the D-genome progenitor of wheat. The library was made in a binary vector that had previously been shown to stably maintain large inserts of foreign DNA in Escherichia coli. The use of root nuclei reduced considerably the proportion of the library containing clones derived from chloroplast DNA. Several experimental parameters were investigated and optimised, leading to a high cloning efficiency. Only three ligations were needed to construct the library which was estimated to be equivalent to 3.7 haploid genomes. The accuracy of this estimation was demonstrated by screening this library with three well-defined probes. One probe containing a glutenin gene sequence identified 5 clones covering at least 230 kb of the Glu-D1 locus and contained the two tightly linked high-molecular-weight glutenin genes Glu-D1x and -D1y. Each of the other two single-copy probes derived from the Cre3 cereal cyst nematode resistance gene locus hybridised with 4 clones containing gene sequences encoding nucleotide binding sites and a leucine-rich region. This is the first representative large-insert DNA library for wheat, and the results indicated that large molecules of wheat DNA can be efficiently cloned, stably maintained and manipulated in a bacterial system.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 75 (1988), S. 599-605 
    ISSN: 1432-2242
    Keywords: T. tauschii ; Chromosomal mapping ; Gliadin ; Glutenin ; Synthetic hexaploid wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In crosses between T. tauschii (D t) accesions, their polymorphic gliadin forms were inherited as blocks of gliadin components -Gli-D t1, Gli-D t2 — as single Mendelian characters. From the progeny of four tri-parental crosses (test-crosses), HMW glutenin subunits derived from T. tauschii (Glu-D t1) segregated as alleles of the Glu-D1 locus in bread wheat. In three of the tri-parental crosses, a small proportion (2.5%) of the progeny with atypical segregation patterns, were identified through somatic chromosome counts, to be aneuploids (1.9% hypoploids and 0.6% hyperploids). Chromosomal mapping studies revealed that the synteny of genes for HMW glutenin subunits and gliadins in T. tauschii are conserved in the D genome ‘homologue’ (chromosome 1D) of T. aestivum. The map distance between the Glu-D1/-D t1 and Gli-D1/-D t1 loci was calculated to be 63.5 cM, while a linkage to the centromere of 7.7–9.7 cM was estimated for the Glu-D1/-D t1 locus.
    Type of Medium: Electronic Resource
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