ISSN:
1617-4623
Keywords:
Key wordsAgaricus bisporus
;
Glutamine synthetase
;
Molecular cloning
;
Gene structure
;
Mushroom
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The gene encoding glutamine synthetase (glnA) was isolated from an Agaricus bisporus H39 recombinant λ phage library. The deduced A. bisporus glutamine synthetase amino acid sequence contains 354 residues. The amino acid sequence is very similar to that derived from the gene coding for glutamine synthetase of the yeast Saccharomyces cerevisiae. The open reading frame is interrupted by four introns. Northern analysis revealed that transcription of the gene is repressed upon addition of ammonium to the culture but the repression was not as strong as that of the gene encoding NADP+-dependent glutamate dehydrogenase (gdhA). Enzyme activities are low in the presence of ammonium, glutamine and albumin and do not correlate with the mRNA levels revealed by Northern analysis. This suggests that glutamine synthetase expression in A. bisporus is also post-transcriptionally regulated by the nitrogen source.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00008612
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