ISSN:
1432-0983
Keywords:
ABF1
;
Glucose-dependent expression TDH3
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Autonomously replicating sequence binding factor 1 (ABF1) has been implicated in the control of a variety of gene expressions in Saccharomyces cerevisiae. In this paper evidence is presented that ABF1 is involved in the glucose-dependent expression of the TDH3 gene which encodes glyceraldehyde-3-phosphate dehydrogenase. ABF1 binds to consensus sites located between-420 and-250, and between +77 and +200, and acts as a transactivator in an orientation-independent manner on both upstream and downstream sites. TDH3-lacZ fusions having an ABF1 consensus motif showed glucose-dependent expression of TDH3, whereas in the abf1 mutant strain JCA35 glucose-dependent expression disappeared. These findings suggest that ABF1 functions as a glucose-dependent transactivator for the expression of the TDH3 gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00352099
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