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  • 1
    Electronic Resource
    Electronic Resource
    Environmental effects on zein chromatograms of maize inbred lines revealed by reversed-phase high-performance liquid chromatography (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 607-612 
    Details
    ISSN: 1432-2242
    Keywords: Taxonomy ; Germplasm identification ; Varietal identity ; Environmental interaction ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Alcohol soluble seed storage proteins (zeins and alcohol soluble glutelins) of maize (Zea mays L.) were separated by reversed-phase high-performance liquid chromatography (RP-HPLC). The objectives were to assess the reproducibility of chromatographic profiles using seed of inbred lines that had been produced in different locations and years. Reproducible differences between sources were seen but these were restricted to proteins that contributed 2% or less to an inbred profile. The majority of variation (93% for peak percent area; 99.8% for elution time) was between inbreds. RP-HPLC can therefore provide distinctive phenotypic profiles that are largely characteristic of genotype. Such qualitative and quantitative data will be valuable for studies of taxonomy, evolution, genetics, and germplasm identification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264264
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  • 2
    Electronic Resource
    Electronic Resource
    Variation in kafirin and alcohol-soluble glutelin chromatograms of sorghum inbred lines revealed by reversed-phase high-performance liquid chromatography (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 97-107 
    Details
    ISSN: 1432-2242
    Keywords: Taxonomy ; Germplasm identification ; Varietal identity ; Environmental interaction ; Genetics ; Multivariate analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Separations of kafirin and alcohol soluble glutelin proteins by reversed-phase high-performance liquid chromatography (RP-HPLC) from 7 inbreds and one hybrid of sorghum [Sorghum bicolor (L.) Moench] and one source of Johnsongrass [Sorghum halapense (L.) Pers.] were compared. Objectives were to assess the stability of protein profiles for seed sources produced at different locations and in different environments to examine the potential of RP-HPLC to provide genotypic profiles for sorghum. Analyses of variance data showed that levels of variation due to environments and locations were small; the majority of variation (93%) was among genotypes. Associations among inbreds revealed by multivariate and cluster analysis showed similarity with those that would be expected on the basis of pedigree. A chi-square analysis showed no deviation in the hybrid profile from the expected 2∶1 ratio of peaks from the female and male inbred parents, respectively. Improvements in the ability to correctly assign common peaks are necessary before associations among numerous sorghum genotypes can be reliably demonstrated by analysis of data from reversed-phase high-performance liquid chromatography (RP-HPLC).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288838
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    Paper (German National Licenses)
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