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  • 1
    Electronic Resource
    Electronic Resource
    Evidence for, and taxonomic value of, an arachidonic acid cascade in the lipomycetaceae (1992)
    Springer
    Antonie van Leeuwenhoek 62 (1992), S. 251-259 
    Details
    ISSN: 1572-9699
    Keywords: arachidonic acid cascade ; Lipomycetaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By using specific inhibitors of the lipoxygenase and cyclo-oxygenase pathways, arachidonic acid metabolites with similar sensitivities towards these inhibitors as in humans, were detected inDipodascopsis uninucleata. The taxonomic value of aspirin sensitive arachidonic acid metabolites in the Lipomycetaceae was next assessed. No metabolites of which the production is inhibited by aspirin were detected in strains representing the following species:Lipomyces starkeyi, Lipomyces kononenkoae, Lipomyces tetrasporus, Myxozyma melibiosi, Myxozyma mucilagina, Myxozyma kluyveri, Waltomyces lipofer, Zygozyma oligophaga andZygozyma arxii. The detection of such aspirin sensitive arachidonic acid metabolites in representative strains ofLipomyces anomalus and the genusDipodascopsis, emphasises the isolated position of these taxa in the genusLipomyces and the family Lipomycetaceae, respectively. Finally using long chain fatty acid analyses, electrophoretic karyotyping and other phenotypic characters, a phylogenetic scheme is proposed for some genera in the Lipomycetaceae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00572592
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  • 2
    Electronic Resource
    Electronic Resource
    The distribution and taxonomic value of fatty acids and eicosanoids in the Lipomycetaceae and Dipodascaceae (1993)
    Springer
    Antonie van Leeuwenhoek 63 (1993), S. 111-123 
    Details
    ISSN: 1572-9699
    Keywords: taxonomy ; fatty acids ; eicosanoids ; Lipomycetaceae ; Dipodascaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using radioimmunoassay, blood platelet aggregation studies and GC-MS the existence of prostaglandins in the endomycetalean yeastDipodascopsis uninucleata was confirmed by our group. These findings triggered the search for similar eicosanoids in the rest of the Endomycetales. We commenced by scanning for the easily detectable precursors of eicosanoids, linoleic- and linolenic acid. We selected two families (i.e. Lipomycetaceae and Dipodascaceae), both producing these precursors, for further investigation. Representative strains of the two families were tested for their ability to grow in the presence of 1mM aspirin, a specific inhibitor of prostaglandin biosynthesis. In contrast to the lipomycetaceous species the dipodascaceous species were insensitive to this drug. These results were verified when representative strains of both families were investigated for their ability to produce eicosanoids from externally fed radio-labeled arachidonic acid along an aspirin sensitive pathway. Thin layer chromatography of culture extracts, followed by autoradiography, showed that while none of the Dipodascaceae produced aspirin sensitive arachidonic acid metabolites, the members of the Lipomycetaceae tested positive for these metabolites. These findings supported the separation of the lipomycetaceous yeastDipodascopsis from the Dipodascaceae. The findings also correlate with the delimitation of these yeasts in two families (i.e. Dipodascaceae and Lipomycetaceae). Further investigation indicated that prostaglandin production by the genusDipodascopsis is mainly associated with ascosporogenesis. Thin layer chromatography of cell extracts fromDipodascopsis tóthii, followed by scintillation counting, indicated the presence of PGF2α and PGE2 during ascosporogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00872387
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  • 3
    Electronic Resource
    Electronic Resource
    Numerical solution of partial differential equations on curved domains by collocation (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Numerical Methods for Partial Differential Equations 9 (1993), S. 357-371 
    Details
    ISSN: 0749-159X
    Keywords: Mathematics and Statistics ; Numerical Methods
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics
    Notes: The application of the finite element collocation method to two- and three-dimensional partial differential equations is hampered by the fact that its accuracy depends largely on the position of the collocation points. The method has, in the past, thus mainly been applied to two-dimensional differential equations defined on rectangular domains. In this case the method yields an optimal global convergence rate, when the Gauss-Legendre quadrature points are used as collocation points. It is shown in this paper that the same accuracy can also be achieved in the case of differential equations defined on nonrectangular domains. The only prerequisite for this is to solve the differential equation on a rectangular domain, mapped onto the nonrectangular domain of the differential equation by a bilinear blended map. © 1993 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/num.1690090403
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  • 4
    Electronic Resource
    Electronic Resource
    The global element method and its extension to parabolic problems (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Numerical Methods for Partial Differential Equations 1 (1985), S. 45-55 
    Details
    ISSN: 0749-159X
    Keywords: Mathematics and Statistics ; Numerical Methods
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics
    Notes: This article describes the derivation of the global element method, originally proposed by Delves and Hall, using the classical Euler-Lagrange theory. The method is then extended so as to be applicable to parabolic partial differential equations. The main advantages of the method, the ease of adjusting its accuracy and applying it to problems with discontinuous coefficients and point singularities, are also discussed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/num.1690010106
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