Publication Date:
2010-05-18
Description:
Staphylococcal superantigen-carrying pathogenicity islands (SaPIs) are discrete, chromosomally integrated units of approximately 15 kilobases that are induced by helper phages to excise and replicate. SaPI DNA is then efficiently encapsidated in phage-like infectious particles, leading to extremely high frequencies of intra- as well as intergeneric transfer. In the absence of helper phage lytic growth, the island is maintained in a quiescent prophage-like state by a global repressor, Stl, which controls expression of most of the SaPI genes. Here we show that SaPI derepression is effected by a specific, non-essential phage protein that binds to Stl, disrupting the Stl-DNA complex and thereby initiating the excision-replication-packaging cycle of the island. Because SaPIs require phage proteins to be packaged, this strategy assures that SaPIs will be transferred once induced. Several different SaPIs are induced by helper phage 80alpha and, in each case, the SaPI commandeers a different non-essential phage protein for its derepression. The highly specific interactions between different SaPI repressors and helper-phage-encoded antirepressors represent a remarkable evolutionary adaptation involved in pathogenicity island mobilization.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518041/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518041/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tormo-Mas, Maria Angeles -- Mir, Ignacio -- Shrestha, Archana -- Tallent, Sandra M -- Campoy, Susana -- Lasa, Inigo -- Barbe, Jordi -- Novick, Richard P -- Christie, Gail E -- Penades, Jose R -- R01AI022159-23A2/AI/NIAID NIH HHS/ -- R21 AI067654/AI/NIAID NIH HHS/ -- R21 AI067654-01A1/AI/NIAID NIH HHS/ -- R21AI067654/AI/NIAID NIH HHS/ -- England -- Nature. 2010 Jun 10;465(7299):779-82. doi: 10.1038/nature09065. Epub 2010 May 16.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Centro de Investigacion y Tecnologia Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Apdo. 187, Segorbe, Castellon 12400, Spain.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20473284" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
Amino Acid Sequence
;
DNA/biosynthesis/genetics
;
DNA Replication
;
Genomic Islands/*genetics
;
Helper Viruses/*enzymology/genetics/metabolism/physiology
;
Lysogeny/physiology
;
Molecular Sequence Data
;
Prophages/metabolism/physiology
;
Pyrophosphatases/chemistry/genetics/metabolism
;
Recombination, Genetic/genetics
;
Repressor Proteins/*antagonists & inhibitors/genetics/metabolism
;
Shock, Septic
;
Staphylococcus Phages/*enzymology/genetics/metabolism/physiology
;
Staphylococcus aureus/*genetics/pathogenicity/virology
;
Superantigens/genetics
;
Up-Regulation/*genetics
;
Viral Proteins/chemistry/genetics/*metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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