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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 14 (1998), S. 565-571 
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; electroporation ; transformation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-fold improvement in transformation frequency was achieved with several mutant strains of S. cerevisiae that transformed poorly by conventional procedures. Both DTT and lithium acetate were necessary for maximal transformation frequencies. Pretreatment with lithium and DTT also resulted in an ∼3·5-fold increase in the electroporation transformation frequency of the pathogenic fungus Candida albicans. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2019-06-27
    Description: A method is presented for using B-splines as potential functions in the estimation of likelihood functions (probability density functions conditioned on pattern classes), or the resulting discriminant functions. The consistency of this technique is discussed. Experimental results of using the likelihood functions in the classification of remotely sensed data are given.
    Keywords: MATHEMATICS
    Type: NASA-CR-140297 , ICSA-275-025-015
    Format: application/pdf
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  • 3
    Publication Date: 2019-07-13
    Description: An approach, based on a global averaging procedure, is presented for estimating the power spectrum of a second order stationary zero-mean ergodic stochastic process from a finite length record. This estimate is derived by smoothing, with a cubic smoothing spline, the naive estimate of the spectrum obtained by applying Fast Fourier Transform techniques to the raw data. By means of digital computer simulated results, a comparison is made between the features of the present approach and those of more classical techniques of spectral estimation.-
    Keywords: MATHEMATICS
    Type: Symposium on Nonlinear Estimation Theory and its Applications; Sep 11, 1972 - Sep 13, 1972; San Diego, CA
    Format: text
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  • 4
    Publication Date: 2019-06-27
    Description: A FORTRAN 4 routine is given which may be used to generate random observations of a continuous real valued random variable. Normal distribution of F(x), X, E(akimas), and E(linear) is presented in tabular form.
    Keywords: MATHEMATICS
    Type: NASA-CR-133983 , ICSA-275-025-007
    Format: application/pdf
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  • 5
    Publication Date: 2019-06-27
    Description: An approach, based on a global averaging procedure, is presented for estimating the power spectrum of a second order stationary zero-mean ergodic stochastic process from a finite length record. This estimate is derived by smoothing, with a cubic smoothing spline, the naive estimate of the spectrum obtained by applying FFT techniques to the raw data. By means of digital computer simulated results, a comparison is made between the features of the present approach and those of more classical techniques of spectral estimation.
    Keywords: MATHEMATICS
    Type: NASA-CR-133986 , ICSA-275-025-004
    Format: application/pdf
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