ISSN:
1432-2242
Keywords:
Genetic instability
;
Somaclonal variation
;
Twin spots
;
Lycopersicon esculentum
;
Tomato
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Among pale-green tomato plants heterozygous for the xanthophyllic2 (xa-2) mutation that were transformed with a T-DNA harbouring the NPTII and GUS gene, a plant with a high frequency of green/white twin spots was found. The genetic analysis of this plant indicated that the occurrence of these twin spots was caused by a genetic defect located at the distal end of chromosome 10S, where xa-2 also is located. The genetic analysis of green plants regenerated from leaf expiants of this twin-spot plant revealed that the green sectors derive from non-disjunction of the xa-2 + allele. In an analysis of mitotic chromosome behaviour bridges were observed in approximately 5% of the anaphases, providing arguments that a breakage-fusion-bridge cycle caused by a tissue culture-induced genomic instability is the most likely cause of this aberrant behaviour of chromosome 10.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00223935
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