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  • 1
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    Identification of DNA sequence responsible for 5-bromodeoxyuridine-induced gene amplification (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-08-31
    Description: Bromodeoxyuridine (BrdUrd) treatment of the prolactin nonproducing subclone of GH cells (rat pituitary tumor cells) induces amplification of a 20-kilobase DNA fragment including all of the prolactin gene coding sequences. This amplified DNA segment, which is flanked by two unamplified regions, thus designates a unit of BrdUrd-induced amplified sequence. Cloned DNA segments, 10.3 kilobases long, from the 5' end of the rat prolactin gene of BrdUrd-responsive and -nonresponsive cells, were ligated to the thymidine kinase gene of herpes simplex virus type 1 (HSV1TK), and the hybrid DNA was transferred to thymidine kinase-deficient mouse fibroblast cells by transfection. The HSV1TK gene and the rat prolactin gene were amplified together in drug-treated transfectants carrying the hybrid DNA HSV1TK gene and rat prolactin gene of BrdUrd-responsive GH cells. These results suggest that the 10.3-kilobase DNA segment at the 5' end of the rat prolactin gene of BrdUrd-responsive GH cells carries the information for drug-induced gene amplification (amplicon) and that another gene, such as the HSV1TK gene, is also amplified when the latter is placed adjacent to this segment.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Biswas, D K -- Hartigan, J A -- Pichler, M H -- CA28218/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1984 Aug 31;225(4665):941-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6089335" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Base Sequence ; Bromodeoxyuridine/*pharmacology ; Cell Line ; Cloning, Molecular ; DNA/*genetics ; DNA, Recombinant ; *Gene Amplification ; Genes, Viral ; Mice ; Prolactin/genetics ; Rats ; Simplexvirus/genetics ; Thymidine Kinase/genetics ; Transfection
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
    Electronic Resource
    Electronic Resource
    The MAP test for multimodality (1994)
    Springer
    Journal of classification 11 (1994), S. 5-36 
    Details
    ISSN: 1432-1343
    Keywords: Minimal constrained spanning trees ; Nearest neighbor density estimates ; Minimal ascending path spanning trees ; Tests for modes ; The MAP test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We introduce a test for detecting multimodality in distributions based on minimal constrained spanning trees. We define a Minimal Ascending Path Spanning Tree (MAPST) on a set of points as a spanning tree that has the minimal possible sum of lengths of links with the constraint that starting from any link, the lengths of the links are non-increasing towards a root node. We define similarly MAPSTs with more than one root. We present some algorithms for finding such trees. Based on these trees, we devise a test for multimodality, called the MAP Test (for Minimal Ascending Path). Using simulations, we estimate percentage points of the MAP statistic and assess the power of the test. Finally, we illustrate the use of MAPSTs for determining the number of modes in a distribution of positions of galaxies on photographic plates from a rich galaxy cluster.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01201021
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