Publikationsdatum:
2008-01-26
Beschreibung:
The propagation of information through signaling cascades spans a wide range of time scales, including the rapid ligand-receptor interaction and the much slower response of downstream gene expression. To determine which dynamic range dominates a response, we used periodic stimuli to measure the frequency dependence of signal transduction in the osmo-adaptation pathway of Saccharomyces cerevisiae. We applied system identification methods to infer a concise predictive model. We found that the dynamics of the osmo-adaptation response are dominated by a fast-acting negative feedback through the kinase Hog1 that does not require protein synthesis. After large osmotic shocks, an additional, much slower, negative feedback through gene expression allows cells to respond faster to future stimuli.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916730/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916730/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mettetal, Jerome T -- Muzzey, Dale -- Gomez-Uribe, Carlos -- van Oudenaarden, Alexander -- 5 R90 DK071511-01/DK/NIDDK NIH HHS/ -- R01 GM068957/GM/NIGMS NIH HHS/ -- R01 GM068957-05/GM/NIGMS NIH HHS/ -- R01 GM068957-06/GM/NIGMS NIH HHS/ -- R01-GM068957/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2008 Jan 25;319(5862):482-4. doi: 10.1126/science.1151582.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18218902" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
*Adaptation, Physiological
;
Cell Nucleus/metabolism
;
*Feedback, Physiological
;
Gene Expression Regulation, Fungal
;
Gene Regulatory Networks
;
Glycerol/*metabolism
;
Mitogen-Activated Protein Kinases/*metabolism
;
Models, Biological
;
Osmolar Concentration
;
Osmotic Pressure
;
Phosphorylation
;
Saccharomyces cerevisiae/genetics/metabolism/*physiology
;
Saccharomyces cerevisiae Proteins/*metabolism
;
Signal Transduction
;
Systems Biology
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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