ISSN:
0173-0835
Keywords:
Keywords: Capillary electrophoresis
;
Endopolygalacturonase
;
Fluorescence labeling
;
Galacturonic acid
;
Substrate specificity
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Slight modifications of published procedures (Stefansson, M. and Novotny, M., Carbohydr. Res. 1994, 258 1-9) allow separation by relative size of 8-aminonaphthalene-1,3,6-trisulfonate (ANTS)-labeled neutral and acidic oligosaccharides by electrophoresis in uncoated capillaries. The separations are performed at pH 2.5 to suppress both any charge from carboxylic acid groups on the oligosaccharides and electroosmotic flow. Utility of the procedure is demonstrated by its application to characterization of substrate specificity of endopolygalacturonases. The results show that both a fungal and a bacterial endopolygalacturonase need four adjacent nonesterified galacturonic acid residues in a pectin to be able to act.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150170215
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