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  • Freeze-substitution  (1)
  • Life and Medical Sciences  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Bacterial mesosomes: Method dependent artifacts (1981)
    Springer
    Archives of microbiology 130 (1981), S. 19-22 
    Details
    ISSN: 1432-072X
    Keywords: Bacillus cereus ; Mesosomes ; Chemical fixation ; Cryofixation ; Freeze-substitution ; Freeze-fracturing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence of mesosomes was investigated during septum formation of vegetative and sporulating cells of Bacillus cereus. It has been demonstrated that bacterial mesosomes which are considered by numerous microbiologists as an integrated constituent of Gram positive bacteria, are in reality artifacts arising during the preparation for electron microscopy. The conventional fixation methods allowed enough time for the cytoplasmic membrane to react to the changed conditions and to form the typical pocket-like membrane invaginations. With cryofixation followed by freeze-substitution it was shown in ultrathin sections that mesosomes do not occur. The extremely rapid freezing and the substitution of the ice by an organic solvent containing the fixative prevented the formation of membraneous artifacts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00527066
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  • 2
    Electronic Resource
    Electronic Resource
    Designing microorganisms for insect control (1985)
    New York, NY : Wiley-Blackwell
    BioEssays 2 (1985), S. 22-25 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Microorganisms belong to the main regulators of insect populations under natural conditions. They perform this function in several ways. In the first place, bacterial metabolites exist which are as potent as chemical insecticides. In the second, entomopathogenic fungi and viruses spread deadly infections among insect populations. The use of advanced techniques in molecular biology, genetic engineering and biotechnology is increasingly expanding the potential of microorganisms as agents of insect pest control.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950020107
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