ISSN:
1432-072X
Keywords:
Key words Cytochrome c biosynthesis
;
Formate-dependent nitrite reduction
;
Disulfide bond
;
formation
;
Escherichia coli anaerobic metabolism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract An Escherichia coli K-12 mutant, isolated on the basis of its inability to catalyze formate-dependent nitrite reduction, was characterized. The mutant was defective in the synthesis of all known c-type cytochromes during anaerobic growth. The mutation was localized by conjugation, transduction, and Southern blotting experiments to the dsbA gene at minute 87 on the E. coli chromosome and was complemented by the wild-type allele. Both DsbA and the recently described DipZ protein were shown to be essential for cytochrome c synthesis, suggesting that they act sequentially in a pathway for cytochrome c assembly in the E. coli periplasm.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002030050268
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