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  • RH-5849  (2)
  • Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Endocrine background of how 20-hydroxyecdysone agonist, RH 5849, influences diurnal pattern of pupation in shape Spodoptera littoralis (1998)
    Springer
    Entomologia experimentalis et applicata 87 (1998), S. 255-261 
    Details
    ISSN: 1570-7458
    Keywords: Spodoptera littoralis ; pesticides ; RH-5849 ; ecdysteroids ; pupation ; circadian rhythmicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Under laboratory conditions (L16:D8, 24°C, r.h. 80%), applying of an ecdysone agonist, RH 5849, (1,2-dibenzoyl-1-tert-butylhydrazine) onto final instar larvae of Spodoptera littoralis (Boisd.), Noctuidae, Lepidoptera, delayed or advanced the time of pupation depending on the time of treatment. This response was dose-dependent. In addition, non-lethal doses of RH 5849, ranging from 0.05 to 10 µg/larva were used. Following treatment of S. littoralis larvae with 5 µg RH 5849 the endogenous ecdysteroid level was increased or decreased depending on the time of application. Based on the results presented, we suggest that potential users of non-steroidal ecdysone agonists should consider the proper circadian timing of application in the field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003258319630
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  • 2
    Electronic Resource
    Electronic Resource
    Nonsteroidal moulting hormone agonists: effects on protein synthesis and cuticle formation in Colorado potato beetle larvae (1999)
    Springer
    Entomologia experimentalis et applicata 93 (1999), S. 1-8 
    Details
    ISSN: 1570-7458
    Keywords: nonsteroidal ecdysone mimic ; RH-0345 ; tebufenozide ; RH-5849 ; precocious moulting ; polypeptide synthesis ; haemolymph ; cuticle ; ultrastructure ; Leptinotarsa decemlineata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Larvae of the Colorado potato beetle, Leptinotarsa decemlineata (Say), that were orally treated with RH-0345 at 0.1 mg l−1, RH-5849 at 10 and 50 mg l−1, tebufenozide at 2 g l−1, and 20-hydroxyecdysone at 2 g l−1, showed symptoms of premature moulting, followed by inhibition of ecdysis. In addition, fresh weight gain and total protein content were blocked. The effects on haemolymphal and cuticular polypeptides after PAGE were linked with premature, new epicuticle deposition as was observed under the electron microscope. These observations support the concept that the ecdysteroid-mimicking action of the three nonsteroidal molecules is specific.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003848728677
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of RH 5849, the first nonsteroidal ecdysteroid agonist, on larvae of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 21 (1992), S. 119-128 
    Details
    ISSN: 0739-4462
    Keywords: toxicity ; physiology ; larval molting ; larval-pupal metamorphosis ; development ; cuticular proteins ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effects of RH 5849 on the larval molt and larval-pupal metamorphosis in Spodoptera littoralis (Boisd.) were examined. Application of RH 5849 to newly ecdysed 3rd and 6th (last) instar larvae induced a larval molt within the first day after application. Symptoms included cessation of feeding and larval weight, extrusion of gut, loss of hemolymph, and a developmentally abnormal and subsequent lethal larval ecdysis. Treated larvae died shortly afterwards. Treated 6th instar larvae, which did not appear to be affected before pupation, showed an abnormal and lethal pupation process.Differences in the protein pattern of the cuticle between treated vs. untreated 6th instar larvae, demonstrated by using PAGE, indicated that in the newly induced larval cuticle some proteins were missing or expressed with less intensity. The lack of several bands in the pattern of cuticular and hemolymph proteins of treated vs. untreated 6th instar larvae, probably from proteins specific for the pupal instar, is suggested as a cause of unsuccessful pupation in the treatment. © 1992 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/arch.940210205
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