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  • Flow injection analysis  (1)
  • Ultrastructure  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 213 (1980), S. 221-235 
    ISSN: 1432-0878
    Keywords: Peripheral blood lymphocyte ; Density separation ; T cell depleted rabbits ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Density separation of purified peripheral blood leucocytes from T-cell depleted rabbits on a linear Ficoll-metrizoate gradient has been applied to obtain different leucocyte fractions. Two lymphocyte fractions separated on density seem to have different characteristics, both morphologically and immunologically. In this study these two fractions have been characterized ultrastructurally by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and a relationship has been established between the surface architecture (SEM), the cell size (SEM/TEM) and surface-Ig/C3-receptors (LM, light microscopy). Finally three types of lymphocytes have been described in the two lymphocyte fractions separated on density. Morphometric information such as cell size, cell shape, eu-/heterochromatin ratio in the nucleus and the nucleus-/cell ratio have been correlated to the stage of activation of the B lymphocyte in a representative density separation.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1040-0397
    Keywords: Modified electrode ; Redox polymer ; Sensor ; Flow injection analysis ; Iron ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The synthesis, characterization, and sensor application of the novel redox polymer [Os(bipy)2(PS)7.5(DMAP)2.5Cl]Cl, where bipy = 2,2′-bipyridyl, PS = polystyrene, and DMAP = poly[4-(N-methyl-N-p-vinylbenzylamino) pyridine], are described. The charge transport properties of electrodes modified with the redox material are investigated using cyclic voltammetry and chronoamperometry. The modified electrode behaves as an efficient electrocatalyst for the outer-sphere reduction of FeIII, with the cross-exchange reaction occurring at the surface of the polymer (Sk″) at concentrations less than 1.0 × 10-3 mol dm-3 FeIII, with a change over to the Ste, kinetic regime at higher substrate concentrations. Direct agreement was observed between the kinetic behavior at rotating disk electrodes and in thin-layer flow cells. Application of the modified electrodes for the determination of iron in pharmaceutical formulations and the long term stability of the electrodes are investigated. The results obtained are compared with those reported for the analogous metallopolymer [Os(bipy)2(PVP)10Cl]Cl, where PVP is poly-4-vinylpyridine.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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