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  • 1
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    In:  http://aquaticcommons.org/id/eprint/22011 | 18721 | 2018-01-22 11:09:49 | 22011 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-06
    Description: In recent years efforts to find bioactive compounds from live organisms especially marine animals have been increased. In the present study, the anticancer and cytotoxic effects of sea cucumber body walls (Holothuria leucospilota) were investigated. For this purpose, sea cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process was done with methanol and diethyl ether solvent which then concentrated by rotary evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells (KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK]) which can be used as potent cytotoxic material. However these extracts did not show significant therapeutic value against KB cells.
    Keywords: Biology ; Fisheries ; Health ; Sea cucumber ; body wall ; methanolic extract ; diethyl etheric extract ; cytotoxic ; anti-cancer ; Holothuria leucospilota ; marine ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 11-20
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  • 2
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    In:  http://aquaticcommons.org/id/eprint/22739 | 18721 | 2018-05-18 22:51:47 | 22739 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-09
    Description: To identify Kraft liquor effects on spermatogenesis and sperm motility of Caspian kutums by using in vitro culture, the fish testis was incubated with 5, 10 and 15 % (V/V) of liquor for 3 and 6 days. The percentage of motile spermatozoa and its total duration were also measured. Adverse effects on testicular cells were more apparent with the increase of liquor concentrations. The mean size of spermatogonia and spermatocytes decreased significantly (p〈0.001) in dose-dependent manner compared to controls. With the increase of concentration, the number of spermatocytes and spermatids decreased (p〈0.001) but the number of spermatogonia, increased (p〈 0.05). The increase of liquor concentration showed a significantly decrease of percentage of motile spermatozoa and total duration of spermatozoa (p〈0.05); but there was no significant difference in most treatments (p〉0.05). This study showed the detrimental effects of low levels of kraft liquor on testicular development and sperm quality in the kutums.
    Keywords: Biology ; Fisheries ; Pollution ; Kutum ; Testis ; Kraft liquor ; Caspian Sea ; Spermatogenesis ; Biology ; Physiology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 918-930
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