Publication Date:
2010-08-21
Description:
Nuclei move to specific locations to polarize migrating and differentiating cells. Many nuclear movements are microtubule-dependent. However, nuclear movement to reorient the centrosome in migrating fibroblasts occurs through an unknown actin-dependent mechanism. We found that linear arrays of outer (nesprin2G) and inner (SUN2) nuclear membrane proteins assembled on and moved with retrogradely moving dorsal actin cables during nuclear movement in polarizing fibroblasts. Inhibition of nesprin2G, SUN2, or actin prevented nuclear movement and centrosome reorientation. The coupling of actin cables to the nuclear membrane for nuclear movement via specific membrane proteins indicates that, like plasma membrane integrins, nuclear membrane proteins assemble into actin-dependent arrays for force transduction.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938394/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938394/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Luxton, G W Gant -- Gomes, Edgar R -- Folker, Eric S -- Vintinner, Erin -- Gundersen, Gregg G -- GM06929/GM/NIGMS NIH HHS/ -- NS059352/NS/NINDS NIH HHS/ -- R01 GM062939/GM/NIGMS NIH HHS/ -- R01 GM099481/GM/NIGMS NIH HHS/ -- R01 NS059352/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 2010 Aug 20;329(5994):956-9. doi: 10.1126/science.1189072.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20724637" target="_blank"〉PubMed〈/a〉
Keywords:
Actins/*metabolism
;
Animals
;
Cell Movement/physiology
;
Cell Nucleus/*metabolism
;
Centrosome/physiology
;
Fibroblasts
;
Lysophospholipids/metabolism
;
Membrane Proteins/metabolism
;
Mice
;
Movement
;
NIH 3T3 Cells
;
Nerve Tissue Proteins/metabolism
;
Nuclear Envelope/*metabolism
;
Nuclear Proteins/*metabolism
;
Telomere-Binding Proteins/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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