ISSN:
1432-072X
Keywords:
Photosynthetic organisms
;
Chlamydomonas reinhardii
;
Ferredoxin-glutamate synthase
;
Ferredoxin-nitrite reductase
;
Common antigenic determinants
;
Ferredoxin-binding domain
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Polyclonal antiserum specific for ferredoxin-nitrite reductase (EC 1.7.7.1) from the green alga Chlamydomonas reinhardii recognized the nitrite reductase from other green algae, but did not cross-react with the corresponding enzyme from different cyanobacteria or higher plant leaves. An analogous situation was also found for ferredoxin-glutamate synthase (EC 1.4.7.1), using its specific antiserum. Besides, the antibodies raised against C. reinhardii ferredoxin-glutamate synthase were able to inactivate the ferredoxin-dependent activity of nitrite reductase from green algae. These results suggest that there exist similar domains in ferredoxin-nitrite reductases and ferredoxin-glutamate synthases from green algae. In addition, both types of enzymes share common antigenic determinants, probably located at the ferredoxin-binding domain. In spite of their physicochemical resemblances, no apparent antigenic correlation exists between the corresponding enzymes from green algae and those from higher plant leaves or cyanobacteria.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00249073
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