Publication Date:
2015-04-25
Description:
An organism with a single recessive loss-of-function allele will typically have a wild-type phenotype, whereas individuals homozygous for two copies of the allele will display a mutant phenotype. We have developed a method called the mutagenic chain reaction (MCR), which is based on the CRISPR/Cas9 genome-editing system for generating autocatalytic mutations, to produce homozygous loss-of-function mutations. In Drosophila, we found that MCR mutations efficiently spread from their chromosome of origin to the homologous chromosome, thereby converting heterozygous mutations to homozygosity in the vast majority of somatic and germline cells. MCR technology should have broad applications in diverse organisms.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687737/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687737/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gantz, Valentino M -- Bier, Ethan -- R01 AI070654/AI/NIAID NIH HHS/ -- R01 AI110713/AI/NIAID NIH HHS/ -- R01 GM067247/GM/NIGMS NIH HHS/ -- R56 NS029870/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 2015 Apr 24;348(6233):442-4. doi: 10.1126/science.aaa5945. Epub 2015 Mar 19.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92095, USA. vgantz@ucsd.edu ebier@ucsd.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25908821" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
Animals
;
Caspase 9
;
*Clustered Regularly Interspaced Short Palindromic Repeats
;
Drosophila melanogaster/genetics
;
Female
;
Genetic Engineering/*methods
;
Genome, Insect
;
Germ Cells
;
*Heterozygote
;
*Homozygote
;
Male
;
*Mutagenesis
;
Mutation
;
Phenotype
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
