ISSN:
1573-0832
Keywords:
Dermatophytosis
;
Feline
;
Immunoblotting
;
M. canis
;
SDS-PAGE
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Water-soluble antigens liberated from the disrupted mycelium of nine dermatophytes (seven isolates ofMicrosporum canis, one each ofMicrosporum gypseum andTrichophyton mentagrophytes) were compared by analytical slab SDS-PAGE. No substantial differences were observed between the protein bands of theM. Canis isolates, but certain distinctive bands were apparent in the other two species examined. Western immunoblotting usingM. canis-derived antigens separated by SDS-PAGE was used to investigate the humoral immune response in 79 cats with naturally-occurring dermatophytosis (72 withM. canis, six withM. gypseum and one withT. mentagrophytes) and this information was compared to results of immunoblots from 46 control (non-dermatophyte exposed) cats. Seven dominant bands (bands which occurred frequently and stained heavily) were identified in immunoblots from the dermatophyte-infected cats with apparent molecular weights varying between 39 and 120 kD. None of these bands were totally specific markers for dermatophytosis as a variable proportion of the control cats showed reactivity to all these proteins. However, most (73%) of the dermatophyte-infected cats showed reactivity to six or seven of the identified bands whereas most (80%) of the control cats showed reactivity to between zero and three of these bands (p〈0.005). Western immunoblotting could be used to select individual immunodominant antigens for further evaluation of protective (cell-mediated) immunity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01103015
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