ISSN:
1432-0827
Keywords:
Extracellular Matrix
;
In Vitro
;
Chondrogenesis
;
Limb bud cells
;
osteogenesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Physics
Notes:
Summary Urea and guanidine extracts of demineralized beef and rabbit bone matrix were assayed bothin vivo andin vitro. One month following intramuscular implantation into mouse thighs, these extracts induced ectopic cartilage and bone. Seven days following continuousin vitro exposure to the same extracts, stage 24 chick limb bud mesenchymal cells in cultures had differentiated into greater numbers of chondrocytes than controls. These results suggest the feasibility of using limb bud mesenchymal cell cultures as anin vitro assay for bone matrix derived, extractable bioactive factors which effect the conversion of mesenchymal cells into chondrocytes as a requisite step inin vivo osteogenesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02405378
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