ISSN:
1435-1536
Keywords:
Key wordsPseudoalteromonas antarctica NF3
;
Exopolymer of glycoproteic character
;
Phosphatidylcholine liposomes
;
Triton X-100
;
Permeability alterations
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract The capacity of the glycoprotein (GP) excreted by Pseudoalteromonas antarctica NF3 to protect phosphatidylcholine (PC) liposomes against the action of Triton X-100 was studied in detail. Increasing amounts of GP assembled with liposomes resulted in a linear increase in the effective surfactant-to-PC molar ratios needed to produce the same alterations in liposomes and in a linear fall in the surfactant partitioning between the bilayer and the aqueous phase. Thus, the higher the proportion of GP assembled with liposomes the lower the surfactant ability to alter the permeability of vesicles and the lower its affinity with these bilayer structures. In addition, increasing GP proportions resulted in a progressive increase in the free surfactant concentration (S W) for the same surfactant–liposome interaction step. The fact that S W was always lower than the surfactant critical micelle concentration indicates that the interaction was mainly ruled by the action of surfactant monomers, regardless of the amount of GP assembled.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s003960050510
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