ISSN:
1617-4623
Keywords:
Escherichia coli K12
;
glpQ gene
;
ugpQ gene
;
Overlapping genes
;
Glycerophosphoryl diester phosphodiesterase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The nucleotide sequences of the glpQ and ugpQ genes of Escherichia coli, which both encode glycerophosphoryl diester phosphodiesterases, were determined. The glpQ gene encodes a periplasmic enzyme of 333 amino acids, produced initially with a 25 residue long signal sequence, while ugpQ codes for a cytoplasmic protein of 247 amino acids. Despite differences in size and cellular location, significant similarity in the primary structures of the two enzymes was found suggesting a common evolutionary origin. The 3′ end of the ugpQ gene overlaps an open reading frame that is transcribed in the opposite direction. This open reading frame encodes a polypeptide with an unusual composition, i.e., 46 of the 146 amino acids are Gln or Asn. This polypeptide and the UgpQ protein were identified in an in vitro transcription/translation system as proteins with apparent molecular weights of 19.5 and 27 kDa, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00273621
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