ISSN:
1612-1112
Keywords:
Gas chromatography
;
Resolution of enantiomers
;
Thermodynamic analysis
;
Enthalpy of interaction
;
Extrapolation of net retention time
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The calculation of capacity factors, k′, from net retention times, t′R, and the corresponding dead times, tM, at different temperatures suffers from the limited accuracy of the tM values. If the temperature coefficient racy of the tM values. If the temperature coefficient d ln k′/d (1/T) only is required, it is sufficient to determine net retention times (t′R)p at constant inlet pressure pi for different temperatures, since the temperature dependence of (tM)p can be assumed as (tM)p=A·eB/T, with B being approximately independent of the column inlet pressure and of the nature of the carrier gas. The extrapolation and interpolation of (t′R)p may be either performed by linear regression or graphically with a nomogram for ln (t′R)p versus 1/T. The resolution factor, α, of two components, e.g. enantiomers which are resolved on a chiral stationary phase, can be treated in a similar way. Examples are given for the resolution of enantiomers of two non-proteinogenic amino acids on the new polysiloxane phase L-Chirasil-CPG.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02290493
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