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  • Engineering General  (1)
  • HDV  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Journal of biomedical science 4 (1997), S. 208-216 
    ISSN: 1423-0127
    Schlagwort(e): HDV ; Ribozyme ; Aminoglycosides ; MgCl2 ; Inhibition ; Chemical modification
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Subgenomic regions of hepatitis delta virus (HDV) RNA contains ribozyme whose activities are important to viral life cycles and depend on a unique pseudoknot structure. To explore the characters of HDV ribozyme, antibiotics of the aminoglycoside, which has been shown inhibiting self-splicing of group I intron and useful in elucidating its structure, were tested for their effect on HDV genomic ribozyme. Aminoglycosides, including tobramycin, netromycin, neomycin and gentamicin effectively inhibited HDV genomic ribozyme self-cleavage in vitro at a concentration comparable to that inhibiting group I intron self-splicing. The extent of inhibition depended upon the concentration of magnesium ion. Chemical modification mapping of HDV ribozyme RNA indicated that the susceptibility of nucleotide 703 to the modifying agent was enhanced in the presence of tobramycin, suggesting a conformational shift of HDV ribozyme, probably due to an interaction with the aminoglycoside. Finally, we examined the effect of aminoglycoside on HDV cleavage and replication in cell lines, however, none of the aminoglycoside effective in vitro exerted suppressive effects in vivo. Our results represented as an initial effort in utilizing aminoglycoside to probe the structure of HDV ribozyme and to compare its reaction mechanism with those of other related ribozymes.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 16 (1992), S. 717-736 
    ISSN: 0363-9061
    Schlagwort(e): Engineering ; Engineering General
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Architektur, Bauingenieurwesen, Vermessung , Geologie und Paläontologie
    Notizen: This paper seeks to reconstruct the parameters of elastic layered media such as P-wave velocity, S-wave velocity, density and thickness from the multioffset seismic reflection data. Since the data are highly non-linear to the low-wavenumber components, the non-linear waveform inversion method, with the aid of generalized ray theory, is proposed to solve this problem in space-time domain. As opposed to the layer-stripping method, the present method attempts to invert all layer parameters simultaneously, thus reducing the cumulative errors resulting from the upper layers. The parameters are inverted by minimizing the weighted square error between the observed data and the calculated data of the layered model, the optimization of which is based on the quasi-Newton method. In synthetic tests, we find that the inverted results are good when the variation of parameters between layers is not too large. The modified method for large variation of parameters is first to fix those of deeper layers and neglect the signals reflected from them, then recover some other parameters simultaneously until those of upper layers attain a stable value, and finally, invert all parameters simultaneously. The results so obtained show a significant improvement. This method was tested to be stable in the presence of noise in seismograms.
    Zusätzliches Material: 17 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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