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  • 1
    Electronic Resource
    Electronic Resource
    On least squares approximations to indefinite problems of the mixed type (1978)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 12 (1978), S. 453-469 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A least squares method is presented for computing approximate solutions of indefinite partial differential equations of the mixed type such as those that arise in connection with transonic flutter analysis. The mehod retains the advantages of finite difference schemes namely simplicity and sparsity of the resulting matrix system. However, it offers some great advantages over finite difference schemes. First, the method is insensitive to the value of the forcing frequency i.e., the resulting matrix system is always symmetric and positive definite,. As a result, iterative methods may be successfully employed to solve the matrix system, thus taking full advantage of the sparsity. Furthermore, the method is insensitive to the type of the partial differential equation, i.e., the computational algorithm is the same in elliptic and hyperbolic regions. In this work the method is formulated and numerical results for model problems are presented. Some theoretical aspects of least squares approximations are also discussed.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620120307
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  • 2
    Electronic Resource
    Electronic Resource
    Sensitivity Of matrix eigenvaluesThis work was done while both authors were summer visitors to Argonne National Laboratory in connection with the LINPACK project. (1978)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 12 (1978), S. 1367-1373 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A recent paper by Donald Gignac describes some difficulties with a certain matrix eigenvalue problem. This paper shows that the difficulties result from the naturue of the matrix itself. Quantities called condition numbers, which measure the sensitivity of the eigenvalues, are computed, and numerical results given.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620120905
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  • 3
    Electronic Resource
    Electronic Resource
    On the density of finite element matrices (1972)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 5 (1972), S. 297-300 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Explicit formulae for the density of a class of finite element matrices are presented. The formulae are functions of the characteristics of the mesh and the element (piecewise polynomial) employed. Results are given for two-dimensional triangular and quadrilateral elements and three-dimensional tetrahedral and cuboid elements. These results are useful in the management of storage in finite element computer programmes.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620050215
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  • 4
    Electronic Resource
    Electronic Resource
    On the numerical solution of free boundary problems (1975)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 9 (1975), S. 239-245 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620090118
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  • 5
    Electronic Resource
    Electronic Resource
    Immunological comparison of desmosomal components from several bovine tissues (1984)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 26 (1984), S. 35-45 
    Details
    ISSN: 0730-2312
    Keywords: desmosome ; immunological analysis ; immunoblotting ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A panel of monoclonal antibodies and conventional antisera directed against desmosomal proteins from bovine muzzle epidermis was used Io identify immunologically related proteins from two other bovine stratified squamous epithelia, cornea and esophagus. Desmosome-enriched tissue fractions were prepared from epidermis, cornea, and esophagus. These tissue extracts were electrophoresed on sodium dodecyl sulfate (SDS)-polyacrylamide gels, blotted onto nitrocellulose paper, and labeled using an indirect immunoperoxidase technique. Labeling with the conventional antisera demonstrates that each of the previously characterized epidermal desmosomal proteins or protein families has an immunologically cross-reacting counterpart in cornea and esophagus. However, chemical differences between homologous desmosomal proteins in these three tissues have also been detected. The corresponding proteins in the different tissues have similar but not always identical apparent molecular weights. Moreover, tissue-restricted antigenic determinants were detected in two of the desmosomal protein families using four monoclonal antibodies, each of which recognizes a distinct antigenic determinant.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240260104
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  • 6
    Electronic Resource
    Electronic Resource
    Properties of a hybrid between lines sensitive and insensitive to contact inhibition of cell divisionAided by grant CA 06793 and postdoctoral fellowship 1-F2-GM-34679 (M. W.) from the United States Public Health Service. (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 71 (1968), S. 105-107 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Hybrid cell lines have been prepared between 3T3, a line highly sensitive to contact inhibition of division, and cl 1-D, an L cell derivative which is not sensitive. A number of hybrid clones isolated were found to be quite sensitive, indicating that in this respect the 3T3 behavior is the more fully expressed in the hybrid. On serial subculture, the hybrid lines gave rise to variants less sensitive to contact inhibition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040710112
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  • 7
    Electronic Resource
    Electronic Resource
    N6-(Δ2-Isopentenyl)adenosine, an inhibitor of cellular transport of uridine and cytidine (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 86 (1975), S. 281-291 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: N6(Δ2-Isopentenyl)adenosine (IPAR) inhibited severely the incorporation of uridine and cytidine into S-180 cells in culture. When IPAR and the nucleosides were simultaneously present in the medium the inhibition was competitive (Ki 3.4 m̈M) and indicated inhibition of transport. However, the inhibition occurred even in the absence of extracellular IPAR if the cells had been preincubated with IPAR. Since 5′-IPAMP was the product which accumulated in large quantities in S-180 cells when incubated with IPAR, the effects of this AMP analog on the intracellular metabolism of uridine had to be considered. No direct correlation between the amount of intracellular IPAMP and the degree of inhibition of uridine utilization was observed and the relative distribution of uridine nucleotides in the acid soluble pool of the cells was unaltered in cells treated with IPAR. Also, IPAMP was not an inhibitor of uridine kinase in a cell free system nor was the activity of this enzyme affected by treatment of cells with IPAR. In addition, a profound inhibition of uridine utilization was also observed in a resistant subline of S-180 cells, which is unable to form IPAMP. These data suggest that IPAMP was not the inhibitory agent. Furthermore, the observation that the inhibition in both sensitive and resistant cells was caused even by a 15-second exposure to 100 m̈M IPAR, followed by rinsing, suggests that IPAR itself is the effective agent.It is concluded that IPAR exerts its inhibitory effect on uridine and cytidine utilization by becoming lodged in the cell membrane and thereby preventing the passage of these nucleosides into the cells. It is also shown that the inhibition of uridine and cytidine utilization by IPAR and by other potent nucleoside uptake inhibitors is unrelated to inhibition of growth or of RNA-synthesis when the cells do not depend on an extracellular source of a nucleoside for growth.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040860212
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  • 8
    Electronic Resource
    Electronic Resource
    Membrane potentials of human fibroblast strains in cultureThis work was supported in part by grants from the U.S. Public Health Service and the Hartford Foundation and USPHS Career Development Award K3-CA 21,230. (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 71 (1968), S. 61-63 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: With a simple technique, the membrane electro-physiology of mammalian fibroblasts in tissue culture can be explored with micro-electrodes. Measured in complete, serum-containing medium, the mean membrane potential for human diploid cell strains in between 70 and 75 millivolts.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040710108
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  • 9
    Electronic Resource
    Electronic Resource
    Development of 3T3-like lines from Balb/c mouse embryo cultures: Transformation susceptibility to SV40 (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 72 (1968), S. 141-148 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Balb/c mouse embryo cultures that are carried under a rigid transfer schedule that minimizes cell-cell contact evolve into permanent lines that possess properties very similar to 3T3. From the same embryo cultures, a transfer schedule where cell-cell contact is extensive leads to lines (Balb/3T12) that form multiple cell layers and have saturation densities up to 25-fold higher than the Balb/3T3 lines. All the lines are hypotetraploid.Comparison of the Balb/3T3 lines with 3T3 reveals similar morphology, ability to grow at high dilution, low saturation density, and high susceptibility to transformation by SV40 virus. The Balb/3T12 lines, which are continually maintained at high cell density, show little improvement in cloning efficiency. Infection with SV40 produces transformants that can be selected by their ability to form colonies at low cell densities. Using the appropriate selective system for each line, it appears that SV40 T antigen induction and transformation in Balb/3T3 and Balb/3T12 are comparable.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040720208
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  • 10
    Electronic Resource
    Electronic Resource
    Sarcoma growth factor (SGF): Specific binding to epidermal growth factor (EGF) membrane receptors (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 102 (1980), S. 267-277 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cells transformed by murine sarcoma viruses (MSV) produce and release into their tissue culture media several polypeptide growth stimulating factors. One of these has been partially purified using Bio-Gel P-60 column chromatography followed by DEAE-cellulose chromatography. This growth factor was assigned the name sarcoma growth factor (SGF), and is here shown to require the epidermal growth factor (EGF) receptor in order to function as a growth factor. DEAE-cellulose chromatography yielded a product that was several-fold purer than the material present in the Bio-Gel P-60 column pool II. The biologically active material from the DEAE-cellulose column, when labeled with 125I, showed specific binding to EGF membrane receptors. The specific binding could be prevented with the addition of either unlabeled EGF or SGF. Both radiolabeled SGF and EGF will bind to live or fixed cells. We were able to bind 125I-SGF as well as 125I-EGF to fixed cells and elute the bound material from fixed receptors. The eluted SGF showed a greater than 25-fold increase in specific binding. The biological activities of EGF and SGF could be bound to and eluted from fixed cells. A 3T3 clone lacking EGF receptors was unable to respond to either EGF or SGF, whereas it responded well to serum and several other purified growth factors. The SGF isolated using DEAE-cellulose chromatography was unable to compete in a radioimmune assay using 125I-EGF and antibody to purified mouse submaxillary gland EGF; it also was not precipitated by anti-EGF antibody.From these studies it appears that the SGF produced and released by these MSV-transformed cells combines with and requires the EGF receptor in order to exert its biological effects. The peptide, however, is antigenically distinct from mouse submaxillary gland EGF.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041020218
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