ISSN:
1432-2048
Keywords:
Cell wall hydrolysis (endosperm)
;
Endosperm (reserve-mannan hydrolysis)
;
Lactuca (seed reserve mobilization)
;
β-Mannoside mannohydrolase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Mobilization of the endosperm cell-wall reserves of Lactuca sativa L. cv. Grand Rapids requires endo-β-mannanase and α-galactosidase activity. A third enzyme, β-mannoside mannohydrolase (EC 3.2.1.25) is also involved. We have determined the optimum extraction and assay conditions for this enzyme, which is soluble only in high-salt (1 M NaCl) buffer. It is located exclusively within the cotyledons, in association with a cellulosic cell-wall fraction. Its substrates are the products of endosperm cell-wall mobilization, mannobiose and mannotriose, which diffuse to the cotyledons and are hydrolyzed extracellularly by the β-mannoside mannohydrolase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392128
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