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  • Endosperm  (2)
  • Geodynamics and Tectonics  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 78 (1989), S. 305-310 
    ISSN: 1432-2242
    Keywords: Endosperm ; Mutant ; Oryza sativa L. ; Protein body ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rice storage proteins of the endosperm are localized in two types of protein bodies, PB-I and PB-II. Protein bodies were isolated by sucrose density gradient centrifugation from developing endosperm of three rice mutants, CM 21, CM 1675 and CM 1834, and characterized after pepsin-digestion treatment by protein contents determination. Mutant protein bodies (PBs) except for their internal structure, were similar in shape and density to PB-I of the variety Kinmaze. Electrophoretic analysis of PB-I polypeptides revealed that SDS (Sodium dodecylsulfate) bands of 13 and 16 kilodaltons consisted, respectively, of four and two individual polypeptides with different pI values, while the 10-kilodalton band behaved as a single polypeptide after isoelectric focussing (IEF) electrophoresis. The differences in the polypeptide composition induced by mutants were due to the decrease and/or increase in the content of specific PB-I polypeptides. Electron microscopic observations revealed that the typical lamellar structure of the PB-I is not visible in CM 1675. On the contrary, the inner portion of PB-I in CM 1834 and CM 21 showed higher electron density than that of the variety Kinmaze. On these two mutants, the content of pepsin-indigestible and -digestible proteins were similar to those of Kinmaze, although the values of the PB-II/PB-I ratio were greater than those for Kinmaze, suggesting that these two mutants are high-glutelin rice mutants.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 76 (1988), S. 11-16 
    ISSN: 1432-2242
    Keywords: Endosperm ; Mutant ; Oryza sativa L. ; Protein body ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To obtain genetic materials to breed qualitatively improved rice storage proteins, we screened about 3,000 mutant lines induced by the treatment of rice fertilized egg cell with N-methyl-N-nitrosourea (MNU). The screening was performed by comparing the profiles of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with that of the original variety, Kinmaze, especially focussing on the changes in polypeptides present in two kinds of protein bodies, PB-I and PB-II. We selected 17 mutant lines and classified them into 4 types on the basis of variations of the relative contents of the polypeptides. Determination of extracted protein in the starchy endosperm of the mutants revealed changes in the content of prolamin and glutelin but not globulin. In some mutants there was marked accumulation of 57 kDa polypeptide concomitant with the remarkable reduction of glutelin subunits. Treatment of the fertilized egg cell with MNU was found to be an effective method to induce mutations for storage proteins in protein bodies of rice.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2016-07-13
    Description: We explore thermal convection of a fluid with a temperature-dependent viscosity in a basally heated 3-D spherical shell using linear stability analyses and numerical experiments, while considering the application of our results to terrestrial planets. The inner to outer radius ratio of the shell f assumed in the linear stability analyses is in the range of 0.11–0.88. The critical Rayleigh number R c for the onset of thermal convection decreases by two orders of magnitude as f increases from 0.11 to 0.88, when the viscosity depends sensitively on the temperature, as is the case for real mantle materials. Numerical simulations carried out in the range of f  = 0.11–0.55 show that a thermal boundary layer (TBL) develops both along the surface and bottom boundaries to induce cold and hot plumes, respectively, when f is 0.33 or larger. However, for smaller f values, a TBL develops only on the bottom boundary. Convection occurs in the stagnant-lid regime where the root mean square velocity on the surface boundary is less than 1 per cent of its maximum at depth, when the ratio of the viscosity at the surface boundary to that at the bottom boundary exceeds a threshold that depends on f . The threshold decreases from 10 6.5 at f  = 0.11 to 10 4 at f  = 0.55. If the viscosity at the base of the convecting mantle is 10 20 –10 21  Pa s, the Rayleigh number exceeds R c for Mars, Venus and the Earth, but does not for the Moon and Mercury; convection is unlikely to occur in the latter planets unless the mantle viscosity is much lower than 10 20  Pa s and/or the mantle contains a strong internal heat source.
    Keywords: Geodynamics and Tectonics
    Print ISSN: 0956-540X
    Electronic ISSN: 1365-246X
    Topics: Geosciences
    Published by Oxford University Press on behalf of The Deutsche Geophysikalische Gesellschaft (DGG) and the Royal Astronomical Society (RAS).
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  • 4
    Publication Date: 2014-12-25
    Description: A series of linear analysis was performed on the onset of thermal convection of highly compressible fluids, in order to deepen the fundamental insights into the mantle convection of massive super-Earths in the presence of strong adiabatic compression. We consider the temporal evolution (growth or decay) of an infinitesimal perturbation superimposed to a highly compressible fluid which is in a hydrostatic (motionless) and conductive state in a basally heated horizontal layer. As a model of pressure-dependence in material properties, we employed an exponential decrease in thermal expansivity α and exponential increase in (reference) density with depth. The linearized equations for conservation of mass, momentum and internal (thermal) energy are numerically solved for the critical Rayleigh number as well as the vertical profiles of eigenfunctions for infinitesimal perturbations. The above calculations are repeatedly carried out by systematically varying (i) the dissipation number (Di), (ii) the temperature at the top surface and (iii) the magnitude of pressure-dependence in α and . Our analysis demonstrated that the onset of thermal convection is strongly affected by the adiabatic compression, in response to the changes in the static stability of thermal stratification in the fluid layer. For sufficiently large Di where a thick sublayer of stable stratification develops in the layer, for example, the critical Rayleigh number explosively increases with Di, together with drastic decreases in the length scales of perturbations both in vertical and horizontal directions. In particular, for very large Di, a thick ‘stratosphere’ occurs in the fluid layer where the vertical motion is significantly suppressed, resulting in a shrink of the incipient convection in a thin sublayer of unstable thermal stratification. In addition, when Di exceeds a threshold value above which a thermal stratification becomes stable in the entire layer, no perturbation is allowed to grow with time regardless of the Rayleigh number and/or the horizontal wavelength. We also found that the effect of adiabatic compression becomes prominent for higher temperature at the top surface of the fluid layer. These findings may imply the crucial importance of adiabatic compression in understanding the dynamics and evolution of the mantles of massive super-Earths, particularly for those orbiting their parent stars very closely.
    Keywords: Geodynamics and Tectonics
    Print ISSN: 0956-540X
    Electronic ISSN: 1365-246X
    Topics: Geosciences
    Published by Oxford University Press on behalf of The Deutsche Geophysikalische Gesellschaft (DGG) and the Royal Astronomical Society (RAS).
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