Publikationsdatum:
2013-06-15
Beschreibung:
Leprosy was endemic in Europe until the Middle Ages. Using DNA array capture, we have obtained genome sequences of Mycobacterium leprae from skeletons of five medieval leprosy cases from the United Kingdom, Sweden, and Denmark. In one case, the DNA was so well preserved that full de novo assembly of the ancient bacterial genome could be achieved through shotgun sequencing alone. The ancient M. leprae sequences were compared with those of 11 modern strains, representing diverse genotypes and geographic origins. The comparisons revealed remarkable genomic conservation during the past 1000 years, a European origin for leprosy in the Americas, and the presence of an M. leprae genotype in medieval Europe now commonly associated with the Middle East. The exceptional preservation of M. leprae biomarkers, both DNA and mycolic acids, in ancient skeletons has major implications for palaeomicrobiology and human pathogen evolution.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Schuenemann, Verena J -- Singh, Pushpendra -- Mendum, Thomas A -- Krause-Kyora, Ben -- Jager, Gunter -- Bos, Kirsten I -- Herbig, Alexander -- Economou, Christos -- Benjak, Andrej -- Busso, Philippe -- Nebel, Almut -- Boldsen, Jesper L -- Kjellstrom, Anna -- Wu, Huihai -- Stewart, Graham R -- Taylor, G Michael -- Bauer, Peter -- Lee, Oona Y-C -- Wu, Houdini H T -- Minnikin, David E -- Besra, Gurdyal S -- Tucker, Katie -- Roffey, Simon -- Sow, Samba O -- Cole, Stewart T -- Nieselt, Kay -- Krause, Johannes -- New York, N.Y. -- Science. 2013 Jul 12;341(6142):179-83. doi: 10.1126/science.1238286. Epub 2013 Jun 13.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Archaeological Sciences, University of Tubingen, 72070 Tubingen, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23765279" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Bone and Bones/microbiology
;
DNA, Bacterial/chemistry/genetics/isolation & purification
;
Denmark
;
Endemic Diseases/history
;
*Evolution, Molecular
;
Genome, Bacterial/*genetics
;
Great Britain
;
History, Medieval
;
Humans
;
Leprosy/epidemiology/history/*microbiology
;
Mycobacterium leprae/*classification/*genetics/isolation & purification
;
Mycolic Acids/chemistry
;
Phylogeny
;
Sweden
;
Tooth/microbiology
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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