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  • 1
    Electronic Resource
    Electronic Resource
    Cryopreservation of encapsulated shoot primordia induced in horseradish (Armoracia rusticana) hairy root cultures (1997)
    Springer
    Plant cell reports 16 (1997), S. 469-473 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cryopreservation ; Encapsulation-dehydration ; Encapsulation-vitrification ; Hairy roots ; Horseradish shoot primordia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot primordia induced in Armoracia rusticana Gaertn. Mey. et Scherb. (horseradish) hairy root cultures were successfully cryopreserved by two cryogenic procedures. Encapsulated shoot primordia were precultured on solidified Murashige-Skoog medium supplemented with 0.5 M sucrose for 1 day and then dehydrated with a highly concentrated vitrification solution (PVS2) for 4 h at 0°C prior to a plunge into liquid nitrogen. The survival rate of encapsulated vitrified primordia amounted to 69%. In a revised encapsulation-dehydration technique, the encapsulated shoot primordia were precultured with a mixture of 0.5 M sucrose and 1 M or 1.5 M glycerol for 1 day to induce dehydration tolerance and then subjected to air-drying prior to a plunge into liquid nitrogen. The survival rate of encapsulated dried primordia was more than 90%, and the revived primordia produced shoots within 2 weeks after plating. A long-term preservation of shoot primordia was also achieved by the technique. Thus, this revised encapsulation-dehydration technique appears promising as a routine method for the cryopreservation of shoot primordia of hairy roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092768
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Cryopreservation of encapsulated shoot primordia induced in horseradish (Armoracia rusticana) hairy root cultures (1997)
    Springer
    Plant cell reports 16 (1997), S. 469-473 
    Details
    ISSN: 1432-203X
    Keywords: Cryopreservation ; Encapsulation-dehydration ; Encapsulation-vitrification ; Hairy roots ; Horseradish shoot primordia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot primordia induced inArmoracia rusticana Gaertn. Mey. et Scherb. (horseradish) hairy root cultures were successfully cryopreserved by two cryogenic procedures. Encapsulated shoot primordia were precultured on solidified Murashige-Skoog medium supplemented with 0.5M sucrose for 1 day and then dehydrated with a highly concentrated vitrification solution (PVS2) for 4 h at 0°C prior to a plunge into liquid nitrogen. The survival rate of encapsulated vitrified primordia amounted to 69%. In a revised encapsulation-dehydration technique, the encapsulated shoot primordia were precultured with a mixture of 0.5M sucrose and 1M or 1.5M glycerol for 1 day to induce dehydration tolerance and then subjected to air-drying prior to a plunge into liquid nitrogen. The survival rate of encapsulated dried primordia was more than 90%, and the revived primordia produced shoots within 2 weeks after plating. A long-term preservation of shoot primordia was also achieved by the technique. Thus, this revised encapsulation-dehydration technique appears promising as a routine method for the cryopreservation of shoot primordia of hairy roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092768
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    Paper (German National Licenses)
    Fulltext
  • 3
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    Correlation between the interlayer Josephson coupling strength and an enhanced superconducting transition temperature of multilayer cuprate superconductors (2012)
    American Physical Society (APS)
    Details
    Publication Date: 2012-02-03
    Description: Author(s): Y. Hirata, K. M. Kojima, M. Ishikado, S. Uchida, A. Iyo, H. Eisaki, and S. Tajima Infrared reflectivity spectra of multilayered HgBa 2 Ca n −1 Cu n O 2 n +2+ δ ( n =2 , 3, 4, and 5) are measured. Optical plasma modes associated with the Josephson coupling between CuO 2 planes within a multilayer appear below T c in the c -axis component of the reflectivity spectrum. The result allows for quantita... [Phys. Rev. B 85, 054501] Published Thu Feb 02, 2012
    Keywords: Superfluidity and superconductivity
    Print ISSN: 1098-0121
    Electronic ISSN: 1095-3795
    Topics: Physics
    Published by American Physical Society (APS)
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  • 4
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    Coexistence of localized and itinerant electrons in BaFe_{2}X_{3} (X=S and Se) revealed by photoemission spectroscopy (2015)
    American Physical Society (APS)
    Details
    Publication Date: 2015-01-14
    Description: Author(s): D. Ootsuki, N. L. Saini, F. Du, Y. Hirata, K. Ohgushi, Y. Ueda, and T. Mizokawa We report a photoemission study at room temperature on BaFe 2 X 3 (X=S and Se) and CsFe 2 Se 3 in which two-leg ladders are formed by the Fe sites. The Fe 2p core-level peaks of BaFe 2 X 3 are broad and exhibit two components, indicating that itinerant and localized Fe 3d sites coexist similar to K x Fe 2−y Se 2 .... [Phys. Rev. B 91, 014505] Published Tue Jan 13, 2015
    Keywords: Superfluidity and superconductivity
    Print ISSN: 1098-0121
    Electronic ISSN: 1095-3795
    Topics: Physics
    Published by American Physical Society (APS)
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    PAPER CURRENT
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