ISSN:
1615-6102
Keywords:
Arachis hypogaea
;
Elicitor-dependent synthesis
;
Nuclear protein
;
Protein synthesis in vivo
;
Solubilization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Pulse labelling experiments with [35S]L-methionine were performed to determine the rate of protein synthesis. Treatment of cultured cells of peanut with fungal cell wall led to a drastic increase in the de novo synthesis of particular proteins in the cytosol, the endoplasmic reticulum and the extracellular compartment. In the nucleus, a single newly synthesized protein, designated p 56, was detectable upon elicitation by fungal elicitor. Pulse labelling with [35S]L-methionine for 1h was applied at various times following elicitation. The time course of p 56 biosynthesis was transient and the maximum of p 56 de novo synthesis preceded the one of the cytosolic protein stilbene synthase. The preferential de novo synthesis and transfer of p 56 to the nucleus, only briefly before the elicitortriggered signal chain causes the activation of nuclear defence genes, makes it a good candidate as member of the signal transduction machinery to the nucleus. p 56 was further characterized by its size as N-octyl-β-D-glucoside micelle. Selective solubilization experiments showed that p 56 is a hydrophobic, not salt extractable protein rather well protected against partial proteolysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01378953
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