ISSN:
1617-4623
Keywords:
Transformation
;
Electroporation
;
Gram-negative bacteria
;
pKT231
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have undertaken a systematic study to test the transformation of various species of gram-negative bacteria using the electroporation method. The data obtained show very clearly that a great variety of gram-negative bacteria — 15 different species belonging to 11 different genera — including freshly isolated wild-type strains can be transformed efficiently by use of the electric-field mediated transformation technique. These include species of the families Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae, photosynthetic bacteria and strains for which transformation could not be achieved, up to now, by other methods.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00332248
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