ISSN:
0173-0835
Keywords:
Organic modifier
;
Micellar electrokinetic chromatography
;
Electroosmotic mobility
;
Electrophoretic mobility of the micelle
;
Mobility ratio
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
The effect of low concentrations of organic modifiers on the electroosmotic mobility, μeo, and electrophoretic mobility of the micelle, μep, mc, in micellar electrokinetic chromatography (MEKC) with sodium dodecyl sulfate (SDS) solutions was investigated. The results showed that μeo was mainly influenced by the viscosity of the running solution. A linear relationship between μeo and the concentration of the organic modifier was found in MEKC. A newly defined parameter, the mobility ratio, Rm, mc, which is the ratio of the electro-phoretic mobility of the micelle to the electroosmotic mobility, and which characterizes the surface charge density of the micelle in MEKC system with organic modifiers, was introduced. A linear relationship between Rm, mc and the organic modifier concentration was observed. The parameter Rm, mc was found to be useful to investigate the changes in the micellar phase with the addition of organic modifier. The measured μep, mc values showed different and interesting characteristics among different organic modifiers.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.11501601241
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