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  • Key words Arbuscular mycorrhiza  (2)
  • Elaeis guineensis  (1)
  • 1
    ISSN: 1432-1890
    Keywords: Key words Arbuscular mycorrhiza ; Hyphal abundance ; Legumes ; Root ; Soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Two arbuscular mycorrhizal (AM) fungi (Glomus mosseae and G. intraradices) were compared for abundance of intraradical and soil-borne hyphae in association with Astragalus sinicum, a small-seeded, and Glycine max, a large-seeded legume. A. sinicum was more responsive than G. max to mycorrhizal formation, especially at early growth stages. Biomass allocation was greater in roots than shoots for mycorrhizal A. sinicum, while the opposite was true for G. max. Hyphal development in root and soil compartments was estimated by trypan blue staining and after staining for succinate dehydrogenase (SDH) or alkaline phosphatase (ALP) activity. Total fungal abundance increased steadily in roots and soil with time to a maximum 8 weeks after planting. SDH- and ALP-active AM hyphae increased in roots during plant growth but decreased in soil at later harvests. Mycorrhizal root mass in A. sinicum and G. max increased about 14-fold and 2.5-fold, respectively, but total length of soil hyphae produced per plant differed little, so that the pattern of AM soil to root abundance of the two fungi varied considerably with the host plant.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1890
    Keywords: Key words Arbuscular mycorrhiza ; Spore nuclei ; Fluorescence in situ hybridization ; Ribosomal DNA loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Fluorescence in situ hybridization (FISH) was applied to interphasic nuclei isolated from spores of four species of AM fungi : Scutellospora castanea, Glomus mosseae, Glomus intraradices and Gigaspora rosea. Ribosomal DNA loci were visualized using digoxigenin-labeled 25 S rDNA probes obtained by nested PCR. Several hybridization sites were detected per nucleus and an internuclear variability was observed in the number of loci. This is the first report of successful application of FISH to analyse the genomes of glomalean fungi.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0789
    Keywords: Vesicular-arbuscular mycorrhizae ; Fertilizers ; Phosphorus ; Acid soils ; Oil palm ; Elaeis guineensis ; Glomus spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of vesicular-arbuscular mycorrhizae on the efficiency of triple superphosphate and rock phosphate fertilizers was compared in two tropical, acid, P-fixing soils (Ivory Coast) in which the available P was labelled with 32PO inf4 sup3- . Both soils were planted with micropropagated oil palms. The growth reponses to the fertilizer applications were low unless accompanied by VAM inoculation, but both fertilizers were equally available to plants. Isotopic-dilution kinetics analyses indicated that the rock phosphate was solubilized in both soils and there was an enrichment of the labile pool of plant-available P, similar to that with superphosphate. The specific activity and the fraction of P derived from either fertilizer was similar in both mycorrhizal and non-mycorrhizal plants, showing that both absorbed P from the same labile pool of P in the fertilized soils. However, VAM inoculation increased the fertilizer utilization coefficient of plants 2.7- to 5.6-fold, depending on the soil and fertilizer. We conclude that VAM inoculation increases fertilizer efficiency, as much of rock phosphate as of superphosphate, for plants growing in acid, P-fixing soils, and the processes involved are not different for the two fertilizers.
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