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  • Ecology  (2)
  • Exotoxin  (2)
  • Fatty acids  (2)
  • 1
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    Isolation and identification of halophilicbacteria from Urmia Lake in Iran (2018)
    Details
    Publication Date: 2021-05-19
    Description: Halophiles are in all three domains of life: Archaea, Bacteria and Eucarya. Halophilic microorganisms in addition to form major part of life biodiversity can have many biotechnological applications. The objective of this research is isolation and identification of halophilic bacteria from Urmia Lake in Iran and the study of its bacterial biodiversity. After sampling of brines from Urmia Lake from 10 stations and depth of approximately 30-50 cm, in April 2011 and transfer to the laboratory in the sterile conditions, samples were enriched and cultured on defined media, and incubated. After appearance of colonies, selected strains were studied based on morphology, physiology and biochemical characteristics. For phylogenetic identification, their genomic DNA were extracted and amplified by PCR technique. Therefore their sequences were determined by genetic experiment based on 16S rRNA gene sequence and their similarity were analysed in GenBank of EzTaxon database. Finally the phylogenetic tree was constructed. Studied strains belonged to three genera: Halomonas 50% (including H. andesensis LC6(T) [12.5%], H. gomseomensis M12(T) [12.5%], H. hydrothermalis Slthf2(T) [12.5%], H. boliviensis LC1(T) [6.25%] and H. janggokensis M24(T) [6.25%]), Salinivibrio 25% (including S.costicolasubsp. alcaliphilus DSM 16359(T) [18.75%] and S. sharmensis BAG(T) [6.25%]) and Idiomarina 25% (including I .loihiensis L2TR(T) [25%]).
    Description: Published
    Keywords: Ecology ; Halophilic bacteria ; Species diversity ; Isolation ; Phylogenetic ; DNA analysis
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.45-59
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  • 2
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    Nanobiosensor designing with molecular framework polymer method compared with agent-linked nanosilica biosensor for Staphylococcus aureus exotoxin detection (2018)
    Details
    Publication Date: 2021-05-19
    Description: Considering the ever increasing population and industrialization leading to developmental trend of humankind's life, we are hardly able to detect the toxins produced in food products using traditional techniques. In this technique, the production of molecular framework and polymer is done using meta acrylic acid monomers, which are formed via covalence connection between meta acrylic acid monomers (MAA) of white polymer. Here also hydrogenised connection between exotoxin amino acid and meta acrylic acid is made that would function as the selective absorption for that. Then in the second stage, based on the bacterial antibody connection to nanoparticle, a sensor was used. In this part of the research, as the basis for absorption for the recognition of bacterial toxin, medium sized silica nanoparticles of 10 nanometer in the form of solid powder were utilized with Notrino brand. Then the suspension produced from agent-linked nanosilica which was connected to bacterial antibody was positioned near the samples of distilled water, that were contaminated with Staphylococcus aureus bacterial toxin with the density of 10-3, so that in case any toxin exists in the sample, a connection between toxin antigen and antibody would be formed. Finally, the light absorption related to the connection of antigen to the particle attached antibody was measured using spectrophotometers. The results indicate that the molecular framework polymer sensor is capable of detecting up to the density of 10-3, but not lower than that, whereas using the second sensor, up to 10-4 of density is detectable. Additionally, the sensitivity of the sensors were examined after 60 days and the first sensor by the day of 28 and the second sensor by the 56 day had confirmatory results and started to decrease after those time periods.
    Description: Published
    Keywords: Biology ; Physiology ; Nanobiosensor ; Staphylococcus aureus ; Exotoxin ; Molecular Framework Polymer Nanosensor
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.667-683
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  • 3
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    Inhibition of fatty acids profile changes of Cobia (Rachycentron canadum) fillets during frozen storage by packaging under vacuum system (2018)
    Details
    Publication Date: 2021-05-19
    Description: This study was aimed to investigate the effect of vacuum packing (VP) on the fatty acids profiles in cobia (Rachycentron canadum) fillets during an extended frozen storage period. Cobia fillets were treated under vacuum system then stored at -18°C for up to 6 months and compared to control conditions. As a result of a frozen storage period of 6 months, a marked content decrease was found in the fatty acid groups such as MUFA, PUFA and ω -3 PUFA, as well as in the ω -3/ ω-6 ratio. However, a preserving effect on such fatty acid parameters could be observed due to the VP treatment. Assessment of the polyene index (PI) indicated an increased lipid oxidation development as a result of the frozen storage time however, this increase was partially inhibited by the vacuum packaging. Results indicate that vacuum packaging was a proper way to reduce lipid oxidation in Cobia fillets and extend their shelf life by omitting available oxygen. Thus the employment of VP alone or in combination with other protective strategies is recommended.
    Description: Published
    Keywords: Fish processing ; Rachycentron canadum ; fillets ; Packaging ; Fatty acids ; Vacuum packaging ; Frozen storage ; Cobia Fish
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.275-288
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  • 4
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    Nanobiosensor designing with molecular framework polymer method compared with agent-linked nanosilica biosensor for Staphylococcus aureus exotoxin detection (2021)
    In:  http://aquaticcommons.org/id/eprint/22717 | 18721 | 2018-05-17 18:27:23 | 22717 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-09
    Description: Considering the ever increasing population and industrialization leading to developmental trend of humankind's life, we are hardly able to detect the toxins produced in food products using traditional techniques. In this technique, the production of molecular framework and polymer is done using meta acrylic acid monomers, which are formed via covalence connection between meta acrylic acid monomers (MAA) of white polymer. Here also hydrogenised connection between exotoxin amino acid and meta acrylic acid is made that would function as the selective absorption for that. Then in the second stage, based on the bacterial antibody connection to nanoparticle, a sensor was used. In this part of the research, as the basis for absorption for the recognition of bacterial toxin, medium sized silica nanoparticles of 10 nanometer in the form of solid powder were utilized with Notrino brand. Then the suspension produced from agent-linked nanosilica which was connected to bacterial antibody was positioned near the samples of distilled water, that were contaminated with Staphylococcus aureus bacterial toxin with the density of 10^-3, so that in case any toxin exists in the sample, a connection between toxin antigen and antibody would be formed. Finally, the light absorption related to the connection of antigen to the particle attached antibody was measured using spectrophotometers. The results indicate that the molecular framework polymer sensor is capable of detecting up to the density of 10^-3, but not lower than that, whereas using the second sensor, up to 10^-4 of density is detectable. Additionally, the sensitivity of the sensors were examined after 60 days and the first sensor by the day of 28 and the second sensor by the 56 day had confirmatory results and started to decrease after those time periods.
    Keywords: Biology ; Fisheries ; Nanobiosensor ; Staphylococcus aureus ; Exotoxin ; Molecular Framework Polymer Nanosensor ; Biology ; Physiology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 667-683
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  • 5
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    Unknown
    Isolation and identification of halophilic bacteria from Urmia Lake in Iran (2021)
    In:  http://aquaticcommons.org/id/eprint/22755 | 18721 | 2018-05-21 17:48:58 | 22755 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-09
    Description: Halophiles are in all three domains of life: Archaea, Bacteria and Eucarya. Halophilic microorganisms in addition to forming a major part of life biodiversity can have many biotechnological applications. The objective of this research is isolation and identification of halophilic bacteria from Urmia Lake in Iran and the study of its bacterial biodiversity. After sampling of brines from Urmia Lake from 10 stations and depth of approximately 30-50 cm, in April 2011 and transfer to the laboratory in the sterile conditions, samples were enriched and cultured on defined media, and incubated. After appearance of colonies, selected strains were studied based on morphology, physiology and biochemical characteristics. For phylogenetic identification, their genomic DNA were extracted and amplified by PCR technique. Therefore their sequences were determined by genetic experiment based on 16S rRNA gene sequence and their similarity were analysed in GenBank of EzTaxon database. Finally the phylogenetic tree was constructed. Studied strains belonged to three genera: Halomonas 50% (including H. andesensis LC6(T) [12.5%], H. gomseomensis M12(T) [12.5%], H. hydrothermalis Slthf2(T) [12.5%], H. boliviensis LC1(T) [6.25%] and H. janggokensis M24(T) [6.25%]), Salinivibrio 25% (including S. costicola subsp. alcaliphilus DSM 16359(T) [18.75%] and S. sharmensis BAG(T) [6.25%]) and Idiomarina 25% (including I. loihiensis L2TR(T) [25%]).
    Keywords: Biology ; Limnology ; Halophilic bacteria ; Species diversity ; Isolation ; Phylogenetic ; DNA analysis ; Urmia Lake ; Ecology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 45-59
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  • 6
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    Unknown
    Inhibition of fatty acids profile changes of cobia (Rachycentron canadum) fillets during frozen storage by packaging under vacuum system (2021)
    In:  http://aquaticcommons.org/id/eprint/22773 | 18721 | 2018-05-21 19:52:14 | 22773 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-10
    Description: This study was aimed to investigate the effect of vacuum packing (VP) on the fatty acids profiles in cobia (Rachycentron canadum) fillets during an extended frozen storage period. Cobia fillets were treated under vacuum system then stored at -18°C for up to 6 months and compared to control conditions. As a result of a frozen storage period of 6 months, a marked content decrease was found in the fatty acid groups such as MUFA, PUFA and ω-3 PUFA, as well as in the ω-3/ ω-6 ratio. However, a preserving effect on such fatty acid parameters could be observed due to the VP treatment. Assessment of the polyene index (PI) indicated an increased lipid oxidation development as a result of the frozen storage time; however, this increase was partially inhibited by the vacuum packaging. Results indicate that vacuum packaging was a proper way to reduce lipid oxidation in Cobia fillets and extend their shelf life by omitting available oxygen. Thus the employment of VP alone or in combination with other protective strategies is recommended.
    Keywords: Chemistry ; Fisheries ; Fatty acids ; Vacuum packaging ; Frozen storage ; Cobia Fish ; Fish processing ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 275-288
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