ISSN:
0749-1581
Keywords:
EPR imaging
;
microtomography
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
EPR imaging and microtomography promises to be a powerful tool for the EPR spectroscopist, but the expansion of this technique was hindered by the lack of commercial apparatus. A simple modification of an existing commercial system that can reach a resolution of the order of 75 μm with a gradient of 150 mT m-1 is described. This resolution can be increased by using larger field gradients. In many biological applications this resolution is significant. As an example, the distribution of a paramagnetic probe in an amphibious egg cell is presented that permits one to distinguish the nucleus of the cell. A summary of the mathematical techniques which enable one to account for the presence of a hyperfine structure in most EPR paramagnetic probes and to increase resolution is also presented.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260331324
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