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  • EPR imaging  (1)
  • Rhizobium  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Extent of high-affinity iron transport systems in field isolates of rhizobia (1994)
    Springer
    Plant and soil 164 (1994), S. 177-185 
    Details
    ISSN: 1573-5036
    Keywords: Rhizobium ; outer-membrane ; siderophores
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A Uruguayan rhizobia collection (67 isolates) obtained from nodules of Medicago sativa, Melilotus albus, Medicago polymorpha, Trifolium subterraneum, Trifolium repens, Trifolium vesiculosum, Lotus corniculatus, Lotus subbiflorus, Lotus pedunculatus, Ornithopus sp. and Adesmia sp. has been examined to assess the occurrence of high affinity iron uptake systems. CAS (Chrome-azurol S)-assay results suggested that most of the free-living form of these microsymbionts may produce siderophores. The highest siderophore production was observed among Medicago and Trifolium microsymbionts whereas no siderophore expression or moderate positive results were found among Lotus microsymbionts; suggesting that microsymbionts of legumes growing on neutral or alkaline soils may express in vitro enhanced siderophore production. Electrophoretic patterns of outer-membrane protein enriched fractions revealed that iron-limited microsymbionts of Medicago sativa, Lotus corniculatus, Lotus subbiflorus, Trifolium repens, Trifolium subterraneum and Ornithopus sp. produced high molecular weight proteins (ranging from 64 to 94 kDa) compared to cells grown in iron-sufficient media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00010069
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  • 2
    Electronic Resource
    Electronic Resource
    EPR imaging in biological applications: Towards microtomography (1995)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 33 (1995), S. S160 
    Details
    ISSN: 0749-1581
    Keywords: EPR imaging ; microtomography ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: EPR imaging and microtomography promises to be a powerful tool for the EPR spectroscopist, but the expansion of this technique was hindered by the lack of commercial apparatus. A simple modification of an existing commercial system that can reach a resolution of the order of 75 μm with a gradient of 150 mT m-1 is described. This resolution can be increased by using larger field gradients. In many biological applications this resolution is significant. As an example, the distribution of a paramagnetic probe in an amphibious egg cell is presented that permits one to distinguish the nucleus of the cell. A summary of the mathematical techniques which enable one to account for the presence of a hyperfine structure in most EPR paramagnetic probes and to increase resolution is also presented.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260331324
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