ISSN:
0935-6304
Keywords:
Amino acids
;
HPLC Light-scatt
;
Light-scattering detection
;
ELSD
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
In this communication the authors report the HPLC SEPARATION OF 20 amino acids without derivatization. Separation was achieved by column swithing using a C-8 reversed-phase column and a cationic ion-exchange column. Detection of the underivatized amino acids was accomplished by means of an evaporative laser light-scattering (ELS) detector. The influence of the temperature of the diffusion tube and nebuolzation gas pressure on detector response is reported. The relationship of peak areas vs. mass show a good log-log linearity within a 1:10 range. Detechtion limit is in the order of 200 pmol for most amino acids under the conditions used.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240200212
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