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  • 1
    Electronic Resource
    Electronic Resource
    Negative regulation of P element excision by the somatic product and terminal sequences of P in Drosophila melanogaster (1993)
    Springer
    Molecular genetics and genomics 237 (1993), S. 145-151 
    Details
    ISSN: 1617-4623
    Keywords: P element ; Transposable elements ; Transposon regulation ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A transient in vivo P element excision assay was used to test the regulatory properties of putative repressor-encoding plasmids in Drosophila melanogaster embryos. The somatic expression of an unmodified transposase transcription unit under the control of a heat shock gene promoter (phsn) effectively repressed P excision in a dose-dependent manner at very low concentrations relative to somatically active transposase (encoded by the hsπΔ2–3 gene). Maximum repression required transcription of the complete transposase gene. Dose-dependent repression of P excision was also observed in the presence of a vector plasmid (pCarnegie4) having only the terminal sequences, including transposase binding sites, of the P element. However, repression required considerably higher concentrations of pCarnegie 4 than phsπ, and elimination of P excision was not observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282795
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  • 2
    Electronic Resource
    Electronic Resource
    P element excision in Drosophila melanogaster and related drosophilids (1991)
    Springer
    Molecular genetics and genomics 225 (1991), S. 387-394 
    Details
    ISSN: 1617-4623
    Keywords: Transposable elements ; P elements ; Excision ; Drosophila melanogaster ; drosophilids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The frequency of P element excision and the structure of the resulting excision products were determined in three drosophilid species, Drosophila melanogaster, D. virilis, and Chymomyza procnemis. A transient P element mobility assay was conducted in the cells of developing insect embryos, but unlike previous assays, this mobility assay permitted the recovery of excision products from plasmids regardless of whether the excision event was precise or imprecise. Both quantitative and qualitative differences between the products of excision in the various species studied were observed. The frequency with which P element excision products were recovered from D. melanogaster was 10-fold greater than from D. virilis and C. procnemis; however, the proportion of all excision events resulting in the reversion of a P-induced mutant phenotype was the same. Virtually all excision products recovered, including those resulting in a reversion of the mutant phenotype, did not result in the exact restoration of the original target sequence. Sequence analysis suggested that duplex cleavage at the 3′ and 5′ termini of the P element, or their subsequent modification, occurred asymmetrically and interdependently. P element-encoded transposase was not absolutely required for P element excision.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261678
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  • 3
    Electronic Resource
    Electronic Resource
    A functional analysis of the P-element gene-transfer vector in insects (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 22 (1993), S. 373-384 
    Details
    ISSN: 0739-4462
    Keywords: gene transformation ; non-drosophilid ; Drosophila melanogaster ; Anastrepha suspensa ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A P-element mobility excision assay was used to determine if non-drosophilid insects could support P gene vector function. Present studies included the testing of Muscids, Sphaerocerids, and Phorids, none of which were able to support P mobility. A new excision indicator plasmid was developed allowing the detection and recovery of virtually all P-element excision products. The frequency and sequence analysis of excision products from Drosophila melanogaster and another drosophilid, Chymomyza procnemis, indicated both quantitative and qualitative differences in the activity of transposase. The quantitative relationships observed in the original assay were maintained, and qualitative differences in transposase activity were reflected in the sequence of the empty donor sites. The results suggest that host factors are involved in cutting and ligating P-element DNA during excision, with transposase facilitating these processes. Possible limitations on P mobility by abnormal transpoase transcript processing were tested in Anastrepha suspensa using transposase-encoding plasmids having deleted intron sequences. A transposase cDNA supported normal P excision in D. Melanogaster, and a low level of mobility in A. suspensa. Possible applications of gene transfer in insects are presented, in particular methods to genetically sterilize and sex insects for the sterile-insect technique. © 1993 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/arch.940220306
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