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  • maize  (3)
  • taxonomy  (3)
  • Dopamine Antagonists/pharmacology
Collection
Keywords
Publisher
Years
  • 1
    Electronic Resource
    Electronic Resource
    A new family of repetitive nucleotide sequences is restricted to the genus Zea
    Amsterdam : Elsevier
    Gene 105 (1991), S. 151-158 
    Details
    ISSN: 0378-1119
    Keywords: Gramineae ; Interspersed repeats ; corn ; evolution ; gene conversion) ; maize ; methylation ; teosinte ; transcribed repeats
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(91)90145-2
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Regulation of the maize HRGP gene expression by ethylene and wounding. mRNA accumulation and qualitative expression analysis of the promoter by microprojectile bombardment (1992)
    Springer
    Plant molecular biology 20 (1992), S. 529-538 
    Details
    ISSN: 1573-5028
    Keywords: cell wall ; ethylene ; genetic transformation ; HRGP ; maize ; wounding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The expression of the maize gene coding for a hydroxyproline-rich glycoprotein (HRGP) has been studied by measuring the mRNA accumulation after wounding or ethylene treatment. RNA blot and in situ hybridization techniques have been used. The temporal and tissue-specific expression has been observed: the cells related to the vascular system show the more intense HRGP mRNA accumulation. Transcriptional constructions of the maize HRGP promoter have been tested on different maize tissues by microbombarding. A 582 bp promoter is able to direct the expression of the gus gene on calli and young leaves. Constructions having shorter promoter sequences lose this ability. The 582 bp construction retains the general specificity of expression observed for the HRGP gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040611
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Subcellular localization of glutelin-2 in maize (Zea mays L.) endosperm (1984)
    Springer
    Plant molecular biology 3 (1984), S. 227-234 
    Details
    ISSN: 1573-5028
    Keywords: endosperm ; immunoelectron microscopy ; glutelins ; maize ; protein blotting ; protein bodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Accumulation of the 28 KD protein of the glutelin-(G2) fraction was followed in developing maize endosperm, using sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and peak integration of scanned gels. 28 KD glutelin-2 could already be observed from 15 days after pollination and its accumulates reached a plateau during the second half of the development period. The process of biosynthesis of 28 KD glutelin-2 and zeins occurs in a parallel way. Subcellular fractions obtained from linear sucrose gradient centrifugation of developing maize endosperms were analyzed by SDS-PAGE and immunoblotting using a serum reacting against glutelin-2 and 14 KD Z2. Glutelin-2 was found to be present in the protein bodies when subcellular fractionation was carried out without dithiothreitol (DTT). The presence of a reducing agent causes the elution of glutelin-2 from protein bodies. Immunocytochemical labelling using the protein A-colloidal gold technique in protein bodies incubated with anti-G2 IgG revealed that G2 is located mainly in the periphery of protein bodies. These results are interpreted as indicating a structural role for glutelins in protein bodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029658
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  • 4
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    Dopamine controls persistence of long-term memory storage (2009)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2009-08-22
    Description: The paradigmatic feature of long-term memory (LTM) is its persistence. However, little is known about the mechanisms that make some LTMs last longer than others. In rats, a long-lasting fear LTM vanished rapidly when the D1 dopamine receptor antagonist SCH23390 was injected into the dorsal hippocampus 12 hours, but not immediately or 9 hours, after the fearful experience. Conversely, intrahippocampal application of the D1 agonist SK38393 at the same critical post-training time converted a rapidly decaying fear LTM into a persistent one. This effect was mediated by brain-derived neurotrophic factor and regulated by the ventral tegmental area (VTA). Thus, the persistence of LTM depends on activation of VTA/hippocampus dopaminergic connections and can be specifically modulated by manipulating this system at definite post-learning time points.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rossato, Janine I -- Bevilaqua, Lia R M -- Izquierdo, Ivan -- Medina, Jorge H -- Cammarota, Martin -- New York, N.Y. -- Science. 2009 Aug 21;325(5943):1017-20. doi: 10.1126/science.1172545.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Centro de Memoria, Instituto do Cerebro, Pontificia Universidade Catolica do Rio Grande do Sul, Porto Alegre, Brazil.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19696353" target="_blank"〉PubMed〈/a〉
    Keywords: 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine/pharmacology ; 8-Bromo Cyclic Adenosine Monophosphate/pharmacology ; Animals ; Benzazepines/pharmacology ; Brain-Derived Neurotrophic Factor/metabolism ; Dopamine/*physiology ; Dopamine Agonists/pharmacology ; Dopamine Antagonists/pharmacology ; Fear ; Hippocampus/drug effects/*physiology ; Male ; Memory/drug effects/*physiology ; Phosphorylation ; Rats ; Rats, Wistar ; Receptors, Dopamine D1/agonists/antagonists & inhibitors/metabolism ; Receptors, N-Methyl-D-Aspartate/metabolism ; Time Factors ; Tyrosine 3-Monooxygenase ; Ventral Tegmental Area/*physiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 5
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    DNA barcoding in Mucorales: an inventory of biodiversity (2013)
    In:  Persoonia - Molecular Phylogeny and Evolution of Fungi (0031-05850) vol.30 (2013) nr.1 p.11
    Details
    Publication Date: 2015-04-20
    Description: The order Mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. To inventory biodiversity in Mucorales ITS barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. Using the ITS sequences, Molecular Operational Taxonomic Units were defined by a similarity threshold of 99 %. An LSU sequence was generated for each unit as well. Analysis of the LSU sequences revealed that conventional phenotypic classifications of the Mucoraceae are highly artificial. The LSU- and ITS-based trees suggest that characters, such as rhizoids and sporangiola, traditionally used in mucoralean taxonomy are plesiomorphic traits. The ITS region turned out to be an appropriate barcoding marker in Mucorales. It could be sequenced directly in 82 % of the strains and its variability was sufficient to resolve most of the morphospecies. Molecular identification turned out to be problematic only for the species complexes of Mucor circinelloides, M. flavus, M. piriformis and Zygorhynchus moelleri. As many as 12 possibly undescribed species were detected. Intraspecific variability differed widely among mucorealean species ranging from 0 % in Backusella circina to 13.3 % in Cunninghamella echinulata. A high proportion of clinical strains was included for molecular identification. Clinical isolates of Cunninghamella elegans were identified molecularly for the first time. As a result of the phylogenetic analyses several taxonomic and nomenclatural changes became necessary. The genus Backusella was emended to include all species with transitorily recurved sporangiophores. Since this matched molecular data all Mucor species possessing this character were transferred to Backusella. The genus Zygorhynchus was shown to be polyphyletic based on ITS and LSU data. Consequently, Zygorhynchus was abandoned and all species were reclassified in Mucor. Our phylogenetic analyses showed, furthermore, that all non-thermophilic Rhizomucor species belong to Mucor. Accordingly, Rhizomucor endophyticus was transferred to Mucor and Rhizomucor chlamydosporus was synonymised with Mucor indicus. Lecto-, epi- or neotypes were designated for several taxa.
    Keywords: Backusella ; biodiversity ; clinical relevance ; DNA barcoding ; intraspecific variability ; ITS ; LSU ; Mucor ; Mucorales ; nomenclature ; Rhizomucor ; taxonomy ; Zygorhynchus
    Repository Name: National Museum of Natural History, Netherlands
    Type: Article / Letter to the editor
    Format: application/pdf
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  • 6
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    DNA barcoding in Mucorales: an inventory of biodiversity (2013)
    In:  Persoonia - Molecular Phylogeny and Evolution of Fungi vol. 30 no. 1, pp. 11-47
    Details
    Publication Date: 2024-01-12
    Description: The order Mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. To inventory biodiversity in Mucorales ITS barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. Using the ITS sequences, Molecular Operational Taxonomic Units were defined by a similarity threshold of 99 %. An LSU sequence was generated for each unit as well. Analysis of the LSU sequences revealed that conventional phenotypic classifications of the Mucoraceae are highly artificial. The LSU- and ITS-based trees suggest that characters, such as rhizoids and sporangiola, traditionally used in mucoralean taxonomy are plesiomorphic traits. The ITS region turned out to be an appropriate barcoding marker in Mucorales. It could be sequenced directly in 82 % of the strains and its variability was sufficient to resolve most of the morphospecies. Molecular identification turned out to be problematic only for the species complexes of Mucor circinelloides, M. flavus, M. piriformis and Zygorhynchus moelleri. As many as 12 possibly undescribed species were detected. Intraspecific variability differed widely among mucorealean species ranging from 0 % in Backusella circina to 13.3 % in Cunninghamella echinulata. A high proportion of clinical strains was included for molecular identification. Clinical isolates of Cunninghamella elegans were identified molecularly for the first time. As a result of the phylogenetic analyses several taxonomic and nomenclatural changes became necessary. The genus Backusella was emended to include all species with transitorily recurved sporangiophores.\nSince this matched molecular data all Mucor species possessing this character were transferred to Backusella.\nThe genus Zygorhynchus was shown to be polyphyletic based on ITS and LSU data. Consequently, Zygorhynchus was abandoned and all species were reclassified in Mucor. Our phylogenetic analyses showed, furthermore, that all non-thermophilic Rhizomucor species belong to Mucor. Accordingly, Rhizomucor endophyticus was transferred to Mucor and Rhizomucor chlamydosporus was synonymised with Mucor indicus. Lecto-, epi- or neotypes were designated for several taxa.
    Keywords: Backusella ; biodiversity ; clinical relevance ; DNA barcoding ; intraspecific variability ; ITS ; LSU ; Mucor ; Mucorales ; nomenclature ; Rhizomucor ; taxonomy ; Zygorhynchus
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
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  • 7
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    A new species concept for the clinically relevant Mucor circinelloides complex (2019)
    Naturalis Biodiversity Center
    In:  Persoonia - Molecular Phylogeny and Evolution of Fungi vol. 44, pp. 67-97
    Details
    Publication Date: 2024-05-02
    Description: Mucor species are common soil fungi but also known as agents of human infections (mucormycosis) and used in food production and biotechnology. Mucor circinelloides is the Mucor species that is most frequently isolated from clinical sources. The taxonomy of Mucor circinelloides and its close relatives (Mucor circinelloides complex – MCC) is still based on morphology and mating behaviour. The aim of the present study was a revised taxonomy of the MCC using a polyphasic approach. Using a set of 100 strains molecular phylogenetic analysis of five markers (ITS, rpb1, tsr1, mcm7, and cfs, introduced here) were performed, combined with phenotypic studies, mating tests and the determination of the maximum growth temperatures. The multi-locus analyses revealed 16 phylogenetic species of which 14 showed distinct phenotypical traits and were recognised as discrete species. Five of these species are introduced as novel taxa: M. amethystinus sp. nov., M. atramentarius sp. nov., M. variicolumellatus sp. nov., M. pseudocircinelloides sp. nov., and M. pseudolusitanicus sp. nov. The former formae of M. circinelloides represent one or two separate species. In the MCC, the simple presence of well-shaped zygospores only indicates a close relation of both strains, but not necessarily conspecificity. Seven species of the MCC have been implemented in human infection: M. circinelloides, M. griseocyanus, M. janssenii, M. lusitanicus, M. ramosissimus, M. variicolumellatus, and M. velutinosus
    Keywords: Ecology ; Evolution ; Behavior and Systematics ; mating tests ; maximum growth temperature ; Mucor ; mucormycosis ; new taxa ; phylogeny ; taxonomy ; zygospore formation
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
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