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Transformation of Aspergillus giganteus to hygromycin B resistance (1990)

Wnendt, Stephan ; Jacobs, Monique ; Stahl, Ulf

Springer

Current genetics 17 (1990), S. 21-24

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ISSN: 1432-0983

Keywords: A. giganteus ; Transformation system ; Dominant selection marker

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary A wild strain of A. giganteus was transformed to hygromycin B resistance using a bacterial resistance gene under the control of A. nidulans sequences. Stable transformants arose by heterogenous integration, mainly of tandem repeats of vector DNA at various sites in the host genome. Between 6 and 30 resistant colonies were obtained per μg DNA per 3×103 viable protoplasts. Vector DNA could be recovered by transformation of Escherichia coli with undigested genomic DNA from Aspergillus giganteus transformants.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00313244

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