ISSN:
1573-5001
Keywords:
Primary amide
;
Stereospecific assignment
;
HMQC spectroscopy
;
Dihedral angle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract An HMQC-based pulse scheme is presented for the stereospecific assignment of asparagineand glutamine side-chain amide protons. The approach makes use of the recently developedquantitative-J correlation spectroscopy [Bax, A. et al. (1994) Methods Enzymol., 239,79–105] to distinguish the E and Z primary amide protons and, as such, eliminates theneed for assignments derived from more time-consuming and potentially ambiguous NOEmethods. An application of this method to a uniformly 15N,13C-labeled cellulose-bindingdomain is presented. When used in combination with a NOESY-HSQC experiment, thepredominant χ2 dihedral angles of two asparagine side chains in this protein can also bedefined.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018635110491
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