ISSN:
1573-0972
Keywords:
Diacetyl reductase
;
enzyme
;
Kluyveromyces marxianus
;
yeast
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Kluyveromyces marxianus had a higher specific activity of diacetyl reductase (EC 1.1.1.5) than all other organisms previously reported. The enzyme was NADH-dependent and irreversibly catalysed the conversion of diacetyl to acetoin with an optimum pH of 7.0. It was stable at 40°C but lost 50% of its activity at 50°C in 30 min. The K m and V max values for diacetyl were 1.8 mm and 0.053 mm/min, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00144456
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