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  • 1
    ISSN: 0173-0835
    Keywords: Densitometry ; Polyacrylamide gel electrophoresis ; Rosaniline dyes ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: In the present study we evaluated the performance of a software/scanner system that employed the Hewlett Packard (HP) ScanJet Plus and Scanplot Software for densitometric quantification of protein loads stained with Coomassie Brilliant Blue following sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Gels with bovine serum albumin (BSA) standards, ranging from 0.125 to 10 μg, were scanned using reflectance densitometry with 127 μm step size in both the x and y directions and a resolution of 200 dots per inch. Densitometric volume was calculated for each protein band from scanner output in the tagged image file format (TIFF) by a customized software package, Scanplot V. 4.05 (Cunningham Engineering). Protein loads between 0.125 and 10.0 μg vs. volume were fit by a second-order regression: Volume = -0.58 × protein load2 + 16.82 × protein load + 7.87 (r = 0.991, p 〈 0.01). The same gels were scanned and quantified using a transmittance laser densitometer; densitometric volumes measured by both systems were highly correlated (r2 = 0.981, p 〈 0.01). Additional gels of BSA, smooth muscle myosin heavy chain (myosin), and actin displayed linear relationships between protein loads up to 4.0 μg and densitometric volume reflecting unique dye binding properties. We conclude that accurate and reproducible quantitative densitometry of SDS-PAGE can be performed using the HP ScanJet Plus scanner and Scanplot software.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2019-07-13
    Description: One of the major problems associated with solid waste processing technologies is effluent contaminants that are released in gaseous forms from the processes. This is a concern in both biological as well as physicochemical solid waste processing. Carbon dioxide (CO2), the major gas released, does not present a serious problem and there are currently in place a number of flight-qualified technologies for CO2 removal. However, a number of other gases, in particular NOx, SO2, NH3, and various hydrocarbons (e.g. CH4) do present health hazards to the crew members in space habitats. In the present configuration of solid waste processing in the International Space Station (ISS), some of these gases are removed by the Trace Contaminant Control System (TCCS), demands a major resupply. Reduction of the resupply can be effective by using catalyst impregnated carbon nanotubes. For example, NO decomposition to N2 and O2 is thermodynamically favored. Data showing decomposition of NO on metal impregnated carbon nanotubes is presented. Comparisons are made of the existing TCCS systems with the carbon nanotube based technology for removing NOx based on mass/energy penalties.
    Keywords: Environment Pollution
    Type: SAE Paper 05ICES-267 , 35th International Conference on Environmental Systems; Jul 11, 2005 - Jul 14, 2005; Rome; Italy
    Format: application/pdf
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  • 3
    Publication Date: 2019-07-13
    Description: Mixed liquid/solid wastes, including feces, water processor effluents, and food waste, can be lyophilized (freeze-dried) to recover the water they contain and stabilize the solids that remain. Our previous research has demonstrated the potential benefits of using thermoelectric heat pumps to build a lyophilizer for processing waste in microgravity. These results were used to build a working prototype suitable for ground- based human testing. This paper describes the prototype design and presents results of functional and performance tests.
    Keywords: Environment Pollution
    Type: SAE Paper TBD , 35th International Conference on Environmental Systems; Jul 11, 2005 - Jul 14, 2005; Rome; Italy
    Format: text
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  • 4
    Publication Date: 2019-07-18
    Description: One of the major problems associated with solid waste processing technologies is the release of effluent gases and contaminants that are in gaseous formed from the processes. A number of other gases, in particular NO(x), SO2, NH3, Hydrocarbons (e.g. CH4) do present hazards to the crew in space habitats. Reduction of mass, power, volume and resupply can be achieved by using catalyst impregnated carbon nanotubes as compared to other catalytic systems. The development and characterization of an innovative approach for the control and elimination of gaseous toxins using single walled carbon nanotubes (SWNTs) promise superior performance over conventional approaches. This is due to the ability to direct the selective uptake of gaseous species based on their controllable pore size, high adsorptive capacity and the effectiveness of carbon nanotubes as catalyst supports for gaseous conversion. For example, SWNTs have high adsorptive capacity for NO and the adsorbed NO can be decomposed to N2 and O2 . Experimental results showing the decomposition of NO on metal impregnated carbon nanotubes is presented. Equivalent System Mass (ESM) comparisons are made of the existing TCCS systems with the carbon nanotube technology for removing NO(x). The potential for methane decomposition using carbon nanotubes catalysts is also discussed.
    Keywords: Environment Pollution
    Type: 35th International Conference on Environmental Systems; Jul 11, 2005 - Jul 14, 2005; Rome; Italy
    Format: text
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