ISSN:
1432-2242
Keywords:
Oryza sativa L.
;
Nitrate reductase
;
Deficient mutant
;
Chlorate resistance
;
Heme domain
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Biochemical and genetical characterization of a rice nitrate reductase (NR)-deficient mutant, M819, which had been isolated as a chlorate-resistant mutant, was carried out. In M819, leaf NADH-NR activity was found to be about 10% of that of the wild-type cv ‘Norin 8’, while NADPH-NR activity was higher than that in the wild-type; FMNH2-NR and MV-NR activities were also 10% of those of the wild type; BPB-NR activity was higher than that of the wild type; and xanthine dehydrogenase activity was revealed to be present in both. These results suggest that the mutant line M819 lacks the functional heme domain of the NADH-NR polypeptide due to a point mutation or a small deletion within the coding region of the structural gene. Chlorate resistance in M819 was transmitted by a single recessive nuclear gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00223974
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