ISSN:
1420-9055
Keywords:
DNA methods
;
Daphnia DNA
;
small-sizedDaphnia
;
zooplankton DNA
;
population variation
;
molecular genetics
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We present methods developed for the small-sizedDaphnia species,D. galeata, D. hyalina, D. cucullata, and their hybrids, which facilitate the use of DNA variation in population studies. Described are large-scale production of material from single genotypes ofDaphnia and subsequent extraction of total DNA. The average DNA yields ranged between 6 µg per gram wet weight (D. cucullata) and 12 µg per gram wet weight (D. galeata). For comparison, the large-sized speciesD. pulex was tested and yielded an average of 28 µg DNA per gram wet weight. The DNA isolated in this manner lends itself well to molecular genetic techniques suited for population studies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00877265
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