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  • DNA synthesis genes  (1)
  • Schizosaccharomyces pombe  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Induction of yeast DNA ligase genes in exponential and stationary phase cultures in response to DNA damaging agents (1986)
    Springer
    Current genetics 11 (1986), S. 107-112 
    Details
    ISSN: 1432-0983
    Keywords: Schizosaccharomyces pombe ; Saccharomyces cerevisiae ; DNA ligase ; DNA damage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary UV-irradiation of stationary phase cells of Saccharomyces cerevisiae and Schizosaccharomyces pombe leads to a 9-fold and 90-fold increase in transcript levels from the respective DNA ligase genes CDC9 and CDC17, whereas exponential cells show only 3-fold and 2-fold increases. Induction of CDC9 after MMS treatment and γ-irradiation was also observed by using a CDC9-lacZ translational fusion and assaying for β-galactosidase. Surprisingly, irradiation of S. cerevisiae induces only a 50% increase in DNA ligase itself, probably reflecting the extremely high in vivo stability of the enzyme. The UV-induction of ligase may be part of a “fail-safe” mechanism which, together with the enzyme stability, ensures adequate supplies of this essential enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00378201
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  • 2
    Electronic Resource
    Electronic Resource
    Budding yeast mutants showing constitutive basal levels of expression of DNA synthesis genes (1993)
    Springer
    Molecular genetics and genomics 240 (1993), S. 36-42 
    Details
    ISSN: 1617-4623
    Keywords: Yeast ; Saccharomyces cerevisiae ; DNA synthesis genes ; Cell cycle regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two mutants have been isolated in Saccharomyces cerevisiae in which transcripts from at least CDC8, CDC9, CDC21 (TMP1) and POL1 genes are expressed constitutively in cells blocked at START by use of either α-pheromone or the cdc28 mutation. The transcripts from these genes also persist in mutant stationary phase cells; however, cell cycle regulation of these four DNA synthesis genes occurs normally in late G1. The mutation therefore does not appear to lie in the MCB-DSC1 (MBF) system that controls the periodic regulation of the genes, but must affect some control mechanism regulating basal levels of expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276881
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