ISSN:
1617-4623
Keywords:
pR plasmid
;
uvp1 gene
;
Recombination
;
DNA repair
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We show that a DNA fragment that contains the uvp1 gene of the plasmid pR directs the synthesis in Escherichia coli minicells of a protein of apparent molecular weight 20 kDa. Inspection of the nucleotide sequence of the region reveals an open reading frame that has the capacity to encode a protein of 198 amino acids. The uvp1 gene product has been found, in two different systems, to enhance the recombination activity of E. coli cells. We have also observed a striking similarity to resolvase and invertase proteins. The significance of this finding for the function of the uvp1 gene product requires further investigation. We conclude that the uvp1 gene encodes a 20 kDa protein which appears to be responsible for enhancement of both UV survival and recominational activity in E. coli.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330560
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